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首页> 外文期刊>American journal of reproductive immunology : >Human chorionic gonadotropin-mediated modulation of pregnancy-compatible peripheral blood natural killer cells in frozen embryo transfer cycles
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Human chorionic gonadotropin-mediated modulation of pregnancy-compatible peripheral blood natural killer cells in frozen embryo transfer cycles

机译：人类绒毛膜促性腺激素介导的妊娠外周血天然杀伤细胞的调节中的冷冻胚胎转移周期

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Problem: To evaluate pregnancy-compatible phenotypic and functional changes in peripheral blood natural killer (pNK) cells during frozen embryo transfer (FET) cycles. Method of study: Peripheral blood was collected from patients undergoing frozen embryo transfer cycles at three separate time points in the cycle. pNK cell phenotype was analyzed by flow cytometry. Impact of pregnancy status on pNK cell cytotoxicity was characterized by two methods: (l)a three-dimensional endovascular tube formation approach and (2) a NK cell-specific K562 cell kill assay. Results: A total of 35 patients were enrolled, 15 with clinical pregnancies and 20 with negative serum β-hCG levels. Overall percentage of CD45~+CD3~-CD56~+ pNK cell did not change during the FET cycle. Pregnancy resulted in an increase in CD45~+CD3~-CD56~+ pNK cell population on the day of serum β-hCG. pNK cells from non-pregnant patients caused significant tube disruption when compared to pregnant patients. Addition of serum from pregnant women reduced the tube disruption by pNK cells from non-pregnant patients. pNK cells from pregnant patients showed significantly lower cytotoxicity toward K562 cells in serum-free conditions. The addition of pregnancy serum decreased non-pregnant pNK cell cytotoxicity. Pregnancy status had no impact on VEGF-A and VEGF-C serum levels. Recombinant hCG added to non-pregnant serum resulted in a significant reduction in non-pregnant pNK cell-mediated K562 cell kill. Conclusion: There was no difference in pNK cell populations based on timing of the FET cycle. However, pregnancy increased the percentage of CD45~+CD3~-CD56~+ pNK cells. Additionally, pNK cells from pregnant women have reduced cytotoxicity and this is possibly mediated by hCG.
机译：问题：在冷冻胚胎转移（FET）周期中，评估外周血自然杀伤（PNK）细胞的妊娠表型和功能变化。研究方法：从周期中三个单独的时间点进行冷冻胚胎转移周期的患者收集外周血。通过流式细胞仪分析PNK细胞表型。怀孕状况对PNK细胞细胞毒性的影响以两种方法为特征：（l）三维血管内管形成方法和（2）NK细胞特异性K562细胞杀伤测定法。结果：共有35例患者，15例临床妊娠，20例血清β-HCG水平阴性。在FET周期中，CD45〜+ CD3〜-CD56〜+ PNK细胞的总比例没有变化。怀孕导致血清β-HCG当天CD45〜+ CD3〜-CD56〜+ PNK细胞群增加。与孕妇相比，非妊娠患者的PNK细胞引起了严重的管破坏。孕妇的血清添加减少了非妊娠患者PNK细胞的管道破坏。在无血清条件下，来自孕妇患者的PNK细胞对K562细胞的细胞毒性明显降低。妊娠血清的添加降低了非妊娠PNK细胞细胞毒性。怀孕状况对VEGF-A和VEGF-C血清水平没有影响。重组HCG添加到非妊娠血清中，导致非妊娠PNK细胞介导的K562细胞杀死显着降低。结论：基于FET周期的时机，PNK细胞群体没有差异。然而，妊娠增加了CD45〜+ CD3〜-CD56〜+ PNK细胞的百分比。此外，来自孕妇的PNK细胞降低了细胞毒性，这可能是由HCG介导的。

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