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首页> 外文期刊>Catalysis science & technology >Facile preparation of MOF-derived MHCo3O4&Co/C with a hierarchical porous structure for entrapping enzymes: having both high stability and catalytic activity
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Facile preparation of MOF-derived MHCo3O4&Co/C with a hierarchical porous structure for entrapping enzymes: having both high stability and catalytic activity

机译：带有分层多孔结构的MOF衍生的MHCO3O4和CO/C的便捷制备，用于捕获酶：具有高稳定性和催化活性

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摘要

MOF-derived porous materials Co3O4 and Co/C with hierarchical structure (MHCo3O4&Co/C) were prepared by the self-sacrificial template strategy, and the surfaces of such materials were functionally modified with a “polydopamine (PDA)” bionic membrane for entrapping horseradish peroxidase (HRP) in this work. MHCo3O4 was prepared under air atmosphere, and Co/C was prepared under nitrogen atmosphere based on the thermolysis of Co-MOF. HRP@PDA@MHCo3O4 and HRP@PDA@Co/C could retain 96.8% and 93.7% catalytic activity compared to the free HRP, respectively, and exhibited better thermal stability and reusability. After 1 h incubation at 70 °C, HRP@PDA@MHCo3O4 and HRP@PDA@Co/C retained 84.8% and 70.8% of the initial activity compared with less than 20% retained by free HRP. After 10 cycles, HRP@PDA@MHCo3O4 and HRP@PDA@Co/C retained 92.4% and 85.4% of the initial activity. Moreover, HRP@PDA@MHCo3O4&Co/C exhibited extreme catalytic degradation efficiency for 2,4-dichlorophenol in simulated wastewater, and 2 mmol L−1 of 2,4-dichlorophenol was completely degraded within 15 min. The superior properties of HRP@PDA@MHCo3O4&Co/C were researched by kinetics and thermodynamics analyses. The results suggested that the mass transfer resistance of the substrates was lower than that of the bulk solution for the reason that the substrates were originally enriched on the hierarchical porous carrier close to the enzyme. The specificity and binding affinity of the enzymatic reactors to substrates were enhanced by the functionalized “PDA” bionic membrane on the carrier surface, compared with free enzyme in bulk buffer.
机译：使用层次结构（MHCO3O4＆CO/C）通过自我分离的模板策略制备了MOF来源的多孔材料CO3O4和CO/C，并用“聚多巴胺（PDA）”在功能上修饰了这种材料的表面这项工作中的过氧化物酶（HRP）。 MHCO3O4是在空气大气下制备的，Co/c是在氮气大气下基于CoMof的热解中制备的。与自由HRP相比，HRP@PDA@MHCO3O4和HRP@pda@co/c可以保留96.8％和93.7％的催化活性，并且表现出更好的热稳定性和可重复性。在70°C下孵育1 h后，HRP@PDA@MHCO3O4和HRP@PDA@CO/C保留了84.8％和70.8％的初始活动，而自由HRP保留的少于20％。 10个周期后，HRP@PDA@MHCO3O4和HRP@PDA@CO/C保留了92.4％和85.4％的初始活动。此外，HRP@PDA@MHCO3O4＆CO/C在模拟废水中显示了2,4-二氯苯酚的极端催化降解效率，在15分钟内完全降解了2,4-二氯苯酚的2 mmol L-1。 HRP@PDA@MHCO3O4和CO/C的出色性质通过动力学和热力学分析研究。结果表明，由于底物最初富集在接近酶的分层多孔载体上，因此底物的传质耐药性低于大体溶液的抗性。与散装缓冲液中的游离酶相比，载体表面的功能化“ PDA”仿生膜增强了酶反应器对底物的特异性和结合亲和力。

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