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首页> 外文期刊>Catalysis science & technology >Biocatalytic characterization of a naturally immobilized lipase found mAraujia sericifera Brot. (Apocynaceae) latex
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Biocatalytic characterization of a naturally immobilized lipase found mAraujia sericifera Brot. (Apocynaceae) latex

机译：天然固定脂肪酶的生物催化表征发现了Maraujia Sericifera brot。 （apocynaceae）乳胶

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摘要

Lipase activity found in the insoluble fraction of Araujia sericifera Brot. (Apocynaceae) (ASL) latex, a native South American milkweed, was characterized by use of different test reactions and under different reaction conditions. In this context, hydrolytic lipase activity towards both natural and synthetic substrates and towards fatty acid esterifications was assayed at different temperatures, pH values, and biocatalyst loads. In the case of natural substrates (cottonseed oil), highest lipase activity was found at pH 8.5 and 60 °C. In the hydrolysis of synthetic substrates (p-nitrophenyl esters) the lipase showed preference for the lowest molecular-weight p-nitrophenyl ester assayed (butyrate). Results of the direct esterification of fatty acids of different chain length in organic media showed that esterification levels of up to 45% could be obtained in one hour of reaction. Activity results were compared with the activity shown by the commercial immobilized lipase Novozym 435 under defined reaction conditions. The high activity exhibited by ASL in the hydrolysis of natural substrates and particularly in the direct esterification of different fatty acids in organic medium, together with its high storage stability, suggests that this plant lipase is a promising biocatalyst for various biotechnological applications.
机译：在Araujia Sericifera Brot的不溶性部分中发现的脂肪酶活性。 （Apocynaceae）（ASL）乳胶是南美本地乳草，其特征是使用不同的测试反应和不同的反应条件下。在这种情况下，在不同的温度，pH值和生物催化剂载荷下测定了对天然和合成底物以及脂肪酸酯的水解脂肪酶活性。在天然底物（棉籽油）的情况下，在pH 8.5和60°C下发现了最高的脂肪酶活性。在合成底物的水解（p-硝基苯酯）中，脂肪酶表现出对最低的分子量P-硝基苯基酯的偏爱（丁酸）。有机培养基中不同链长的脂肪酸直接酯化的结果表明，在一个小时的反应中，可以获得高达45％的酯化水平。将活性结果与定义的反应条件下的商业固定脂肪酶Novozym 435所示的活性进行了比较。 ASL在自然底物的水解中表现出的高活性，尤其是在有机培养基中不同脂肪酸的直接酯化以及其高储存稳定性中，这表明该植物脂肪酶是各种生物技术应用的有希望的生物催化剂。

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