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首页> 外文期刊>Catalysis science & technology >Preparation of p-mannanase CLEAs using macromolecular cross-linkers
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Preparation of p-mannanase CLEAs using macromolecular cross-linkers

机译:使用大分子交联剂制备P-甘露酶Cleas

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摘要

Cross-linked enzyme aggregates (CLEAs) of p-mannanase were prepared by precipitation and subsequent cross-linking. The macromolecular cross-linkers (dialdehyde starch and dextran polyaldehyde) with different molecular weight were developed instead of traditional glutaraldehyde so as to improve the activity of CLEAs toward macromolecular substrates. Scanning electron microscopy revealed that CLEAs prepared using linear dextran polyaldehyde (M_w = 2000 kDa) presented a porous structure with low steric hindrance, and thus exhibited excellent activity toward macromolecular substrates, which was 16 times higher than that prepared using glutaraldehyde.
机译:通过沉淀和随后的交联制备P-甘露酶的交联酶聚集体(CLEA)。 开发了具有不同分子量的大分子交联(二醛淀粉和葡萄糖多醛),而不是传统的戊二醛,从而改善了cleas的活性。 扫描电子显微镜表明,使用线性葡萄糖多醛(M_W = 2000 kDa)制备的CLEA提出了一种具有低正位阻滞的多孔结构,因此表现出极好的活性,对大分子分子底物表现出极好的活性,比使用glutararaldehyde制备的质量是16倍。

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