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首页> 外文期刊>Otology and neurotology: official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology >Neurotrophic receptors as potential therapy targets in postnatal development, in adult, and in hearing loss-affected inner ear.
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Neurotrophic receptors as potential therapy targets in postnatal development, in adult, and in hearing loss-affected inner ear.

机译：神经营养受体作为产后发育，成人和受听力损失影响的内耳的潜在治疗靶标。

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HYPOTHESIS: : The aim of this investigation was to define the expression of neurotrophic receptors within the developing inner ear of different postnatal ages. BACKGROUND: : Pattern of differential expression of neurotrophic receptors provide molecular target sites for multifunctional nanoparticle-based cell-specific therapeutics delivery to treat hearing diseases. METHODS: : Protein expression of neurotrophic receptors was studied by immune-histochemistry, quantitative polymerase chain reaction, in situ hybridization, Western blot, in early and late postnatal, adult, and aging mice. RESULTS: : There was a high correlation between results obtained at ribonucleic acid and protein levels. TrkB and TrkC gene expression increased during the first 2 weeks and also after the onset of hearing in adult mice. At the onset of hearing, TrkB-immunopositive staining occurred in inner hair cells and in cell bodies of spiral ganglion neurons. TrkC was detected in nerve endings beneath inner and outer hair cells and in supporting cells. Root cells within the spiral ligament and spiral ganglion neurons in the Rosenthal's canal showed high level of TrkC expression. p75NTR was found in organ of Corti similar to TrkC, and scattered neurons showed strong immunoreactivity in the Rosenthal's canal. PD540 mice, a model of age-related hearing loss, showed a complete spiral ganglion cell loss in the basal turn. Although TrkB and TrkC were completely lacking in this region of the Rosenthal's canal, remaining nerve fibers were p75NTR immunopositive. CONCLUSION: : We found differential expression pattern of TrkB, TrkC, and p75NTR receptors in the inner ear and could make a receptor expression data base. These findings, in turn, will help to design a study on receptor-specific drug targeting of the mice model of auditory development and aging.
机译：假设：：这项研究的目的是定义不同产后年龄发展的内耳内神经营养受体的表达。背景：：神经营养受体差异表达的模式为基于纳米颗粒的细胞特异性治疗剂提供分子靶位，以治疗听力疾病。方法：：通过免疫 - 归化，定量聚合酶链反应，原位杂交，蛋白质印迹，在产后和晚期，成人和衰老小鼠中研究神经营养受体的蛋白质表达。结果：：核糖核酸和蛋白质水平的结果之间存在很高的相关性。 TRKB和TRKC基因表达在最初的两周以及成年小鼠的听力发作后也有所增加。听力开始时，内毛细胞和螺旋神经节神经元的细胞体发生了TRKB免疫阳性染色。在内部和外毛细胞下以及支持细胞的神经末端检测到TRKC。罗森塔尔管中的螺旋韧带和螺旋神经神经元内的根细胞显示出高水平的TRKC表达。在类似于TRKC的Corti器官中发现了P75NTR，散射的神经元在Rosenthal的运河中显示出强烈的免疫反应性。 PD540小鼠是与年龄相关的听力损失模型，在基础转弯中显示出完全的螺旋神经节细胞损失。尽管Rosenthal运河的这一区域完全缺乏TRKB和TRKC，但仍保持神经纤维是P75NTR免疫阳性。结论：：我们发现内耳中TRKB，TRKC和P75NTR受体的差异表达模式，并且可以形成受体表达数据库。反过来，这些发现将有助于设计有关受体​​特异性药物靶向听觉发展和衰老模型的研究。

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