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The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus

机译:的传播、量化和存储水泡性口炎病毒

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Vesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative‐sense single‐stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platformand an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also review the proper safety protocol for the handling of VSV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention.
机译:水泡性口炎病毒(VSV)典型的Rhabdoviridae家族的成员负的量意义单一链RNA病毒。这种病毒已经作为一个强大的模型系统几十年,目前正在被用作疫苗platformand溶瘤细胞的代理。我们提出的方法来传播、量化和水疱性口炎病毒商店。水疱性口炎病毒的协议处理,归类为2病原生物安全水平的实验室美国疾病控制中心预防。

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