Yersinia pseudotuberculosis: Cultivation, Storage, and Methods for Introducing DNA

Robert K. Davidson; Kimberly M. Davis

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Yersinia pseudotuberculosis: Cultivation, Storage, and Methods for Introducing DNA

机译：鼠疫的伪:种植、储存、和方法引入DNA

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Yersinia pseudotuberculosis has been studied for many decades, and research on this microbe has taught us a great deal about host‐pathogen interactions, bacterial manipulation of host cells, virulence factors, and the evolution of pathogens. This microbe should not be cultivated at 37°C because this is a trigger that the bacterium uses to sense its presence within a mammalian host and results in expression of genes necessary to colonize a mammalian host. Prolonged growth at this temperature can result in accumulation of mutations that reduce the virulence of the strain, so all protocols need to be modified for growth at room temperature, or 26°C. This article describes protocols for cultivating this microbe and for its long‐term storage and itsgenetic manipulation by transformation and conjugation.

机译：鼠疫的伪已经研究了几十年,这种微生物的研究教给我们很多关于主机的病原体交互,细菌操纵主机细胞毒性因子的进化病原体。在37°C,因为这是一个引发的细菌使用中感觉它的存在哺乳动物宿主和结果表达的基因有必要在哺乳动物宿主。在这个温度会导致经济增长突变积累减少菌株的毒力,所以所有的协议都需要被修改的增长在室温或26°C。培养微生物和长期存储和操纵itsgenetic转换和接合。

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《Current Protocols in Microbiology》 |2020年第1期|Yersinia pseudotuberculosis/-Yersinia pseudotuberculosis/19|共19页
作者
Robert K. Davidson; Kimberly M. Davis;
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作者单位

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland;

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正文语种英语
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Virulence; Yersinia pseudotuberculosis; conjugationMicrobesStoragebacteria;

机译：毒性;鼠疫的伪;conjugationM;

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1. Experimental infection with Yersinia pseudotuberculosis in European brown hare (Lepus europaeus, Pallas) [J] . Filippo Fratini, Ranieri Verin, Valentina V. Ebani, 亚太热带医药杂志（英文版） . 2017,第3期
2. Optimized Methods for Biofilm Analysis in Yersinia pestis [J] . FANG Nan, GAO He, WANG Li, 生物医学与环境科学（英文版） . 2013,第005期
3. Assessment of Cultivation Method for Energy Beet Based on LCA Method [J] . Chunfeng, ZHANG, Feng, 亚洲农业研究：英文版 . 2014,第002期
4. Carbon storage in biomass,litter,and soil of different native and introduced fast-growing tree plantations in the South Caspian Sea [J] . Jamshid Eslamdoust, Hormoz Sohrabi 林业研究（英文版） . 2018,第002期
5. Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica [J] . José-Antonio Bengoechea, Buko Lindner, Ulrich Seydel, Microbiology . 1998,第6期

机译：yersinia pseudotuberculosis和yersinia pestis比yersinia肠菌属更耐受杀菌阳离子肽
6. Bioluminescent tracing of a Yersinia pestis pCD1(+)-mutant and Yersinia pseudotuberculosis in subcutaneously infected mice [J] . Zhou Yazhou, Zhou Jiyuan, Ji Yuxin, Microbes and infection . 2018,第3期

机译：yersinia pestis pcd1（+） - 突变体和yersinia pseudotuberculosis的生物发光追踪在皮下感染的小鼠
7. The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is active against cultured mammalian cells [J] . Michelle C. Hares, Stewart J. Hinchliffe, Philippa C. R. Strong, Microbiology . 2008,第11期

机译：yersinia pseudotuberculosis和yersinia pestis毒素复合物对培养的哺乳动物细胞有效
8. Yersinia pestis and Yersinia pseudotuberculosis diversity in the former Soviet Union and Mongolia [C] . Andrey P. Anisimov International symposium on bacterial genomics, evolution and pathogenesis . 2010

机译：yersinia pestiis和yersinia pseudotuberculosis多样性在前苏联和蒙古
9. Oral Vaccination with LcrV from Yersinia pestis KIM delivered by live attenuated Salmonella enterica serovar Typhimurium elicits a protective immune response against challenge with Yersinia pseudotuberculosis and Yersinia enterocolitica [O] . Christine G. Branger, Ascención Torres-Escobar, Wei Sun, -1

机译：来自yersinia pestis Kim的Lcrv口腔疫苗接种extenica serovar毒蕈鼠塞洛维尔毒蕈毒蕈ryphimurium elricits针对yersinia pseudotuberculosis和yersinia肠梗解释的保护性免疫应答
10. Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica [O] . José-Antonio Bengoechea, Buko Lindner, Ulrich Seydel, 1998

机译：yersinia pseudotuberculosis和yersinia pestis比yersinia肠菌属更耐受杀菌阳离子肽
11. Novel Protein, LcrQ, Involved in the Low-Calcium Response of 'Yersinia pseudotuberculosis' Shows Extensive Homology to YopH. [R] . Rimpilaeinen, M., Forsberg, A., Wolf-Watz, H. 1992

机译：新型蛋白质，LcrQ，参与'Yersinia pseudotuberculosis'的低钙反应显示广泛的同源性YopH。

Yersinia pseudotuberculosis: Cultivation, Storage, and Methods for Introducing DNA

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