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Purification of Yeast Spores to Investigate Their Dynamics of Activation

机译:净化的酵母孢子对他们进行调查动态激活

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摘要

Germination is an important developmental process that supports resumption of growth in dormant spores. The study of the mechanisms underlying germination requires a pure spore population devoid of other cell types. This article describes the sporulation of wild Saccharomyces cerevisiae and Saccharomyces paradoxus strains, and the isolation and purification of ascospores. We also describe a method to synchronously induce germination in a spore population as well as to measure spore activation. This procedure can be applied, for example, to the study of environmental conditions that trigger germination.
机译:发芽是一个重要的发展过程支持休眠恢复增长孢子。萌发需要一个纯粹的孢子数量没有其他细胞类型。描述了野生酵母属的孢子形成酵母和酿酒脉菌株,和子囊孢子的隔离和净化。我们还描述同步诱导方法在孢子萌发以及人口测量孢子激活。应用,例如,研究环境条件触发发芽。

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