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Ixodid Tick Dissection and Tick Ex Vivo Organ Cultures for Tick‐Borne Virus Research

机译:Ixodid蜱虫解剖和蜱虫体外器官文化对蜱虫承担应承担的病毒研究

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摘要

Tick‐borne viruses cause thousands of cases of disease worldwide every year. Specific countermeasures to many tick‐borne viruses are not commercially available. Very little is known regarding tick‐virus interactions and increasing this knowledge can lead to potential targets for countermeasure development. Virus infection of ex vivo organ cultures from ticks can provide an approach to identify susceptible cell types of tissue to infection. Additionally, these organ cultures can be used for functional genomic studies to pinpoint tick‐specific genes involved in the virus lifecycle. Provided here are step‐by‐step procedures to set up basic tick organ cultures in combination with virus infection and/or functional genomic studies. These procedurescan be adapted for future use to characterize other tick‐borne pathogen infections as well as tick‐specific biological processes.
机译:蜱虫承担应承担的病毒导致数千例每年全球疾病。许多蜱虫病毒承担应承担的对策不是商用。有关蜱虫病毒相互作用和增加这些知识可能导致潜在的目标发展对策。活体器官从蜱虫可以提供一个文化识别易感细胞类型的方法组织感染。文化可以用于功能基因组研究查明蜱虫的特定基因病毒的生命周期。由必经的一步一步检测程序建立基本的蜱虫机关文化结合病毒感染和/或功能基因组研究。这些procedurescan被改编为将来使用描述其他蜱虫承担应承担的病原体感染以及地理蜱虫生物过程。

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  • 来源
    《Current Protocols in Microbiology》 |2020年第1期|Ixodid Tick Dissection/1-Ixodid Tick Dissection/19|共19页
  • 作者单位

    Biology of Vector‐Borne Viruses Section, Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana;

    Visual Medical Arts, Research and Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana;

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  • 原文格式 PDF
  • 正文语种 英语
  • 中图分类
  • 关键词

    EX-VIVO; viruses; Virus DiseasesOrgan cultureTickscell type;

    机译:DiseasesOrgan体外;病毒;病毒cultureTickscell类型;

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