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首页> 外文期刊>Current Protocols in Microbiology >Dissecting the Biology of the Fungal Wheat Pathogen Zymoseptoria tritici: A Laboratory Workflow
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Dissecting the Biology of the Fungal Wheat Pathogen Zymoseptoria tritici: A Laboratory Workflow

机译：解剖小麦真菌的生物学病原体Zymoseptoria tritici:一个实验室工作流

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摘要

The fungus Zymoseptoria tritici is one of the most devastating pathogens of wheat. Aside from its importance as a disease‐causing agent, this species has emerged as a powerful model system for evolutionary genetic studies of crop‐infecting fungal pathogens. Z. tritici exhibits exceptionally high levels of genetic and phenotypic diversity as well as morphological plasticity, which can make experimental studies and comparability of results obtained in different laboratories, e.g., from infection assays, challenging. Therefore, standardized experimental methods are crucial for research on Z. tritici biology and the interaction of this fungus with its wheat host. Here, we describe a suite of well‐tested and optimized protocols ranging from isolationof Z. tritici field specimens to analyses of virulence assays under controlled conditions. Several biological and technical aspects of working with Z. tritici under laboratory conditions are considered and carefully described in each protocol.
机译：真菌Zymoseptoria tritici是最之一毁灭性的病原体的小麦。作为一个疾病导致代理重要性,这一点物种已经成为一个强大的模型系统进化遗传学研究农作物感染真菌病原体。展览非常高水平的遗传和表型多样性以及形态可塑性,从而使实验研究和结果的可比性不同的实验室,例如,从感染化验,有挑战性。实验研究方法是至关重要的z tritici生物学和交互小麦主机的真菌。套的测试和优化的协议从isolationof z tritici字段标本分析下的毒力测定控制条件。的技术方面和z . tritici一起工作在实验室条件下被认为是和仔细描述在每个协议。

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