An Exonuclease V–qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Lines and Tissues

Julia E. Myers; Katarzyna Zwolinska; Martin J. Sapp

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An Exonuclease V–qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Lines and Tissues

机译：一个核酸外切酶V-qPCR化验分析状态人类乳头瘤病毒16基因组的细胞行和组织

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Integration of the human papillomavirus (HPV) genome into host cell chromosomes has been observed in a majority of HPV‐positive cervical cancers and a subset of oral HPV‐associated cancers. HPV integration also occurs in long-term cell culture. Screening for HPV integration can be labor intensive and yield results that are difficult to interpret. Here we describe an assay based on exonuclease V (ExoV/RecBCD) and quantitative polymerase chain reaction (qPCR) to determine if samples from cell lines and tissues contain episomal or integrated HPV. This assay can be applied to screen other small DNA viruses with episomal/linear genome configurations in their viral lifecycle and has the potential to be used in clinical settings to define viral genomic conformations associated with disease.

机译：人类乳头状瘤病毒(HPV)的集成基因组到宿主细胞的染色体观察颈多数人乳头状瘤病毒检测阳性癌症和口腔人乳头状瘤病毒检测相关的一个子集癌症。细胞培养。劳动密集型和产生的结果很难解释。基于V (ExoV / RecBCD)和核酸外切酶定量聚合酶链反应(qPCR)确定样本细胞系和组织含有游离或集成人乳头状瘤病毒。可以应用于屏幕其他小的DNA病毒吗与游离/线性基因组配置病毒生命周期和有潜力在临床设置用于定义病毒基因组构象与疾病有关。

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来源
《Current Protocols in Microbiology》 |2020年第1期|An Exonuclease/1-An Exonuclease/15|共15页
作者
Julia E. Myers; Katarzyna Zwolinska; Martin J. Sapp;
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Department of Microbiology and Immunology, Louisiana State University Health Sciences Center-Shreveport, Shreveport, Louisiana;

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正文语种英语
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关键词
Cell Line; Human Papilloma Virus; Human papillomavirus 16TissuesExonucleasesSubsetHost CellGenome;

机译：细胞系;人类乳头状瘤病毒;人类CellGenome;

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1. CLONING OF HUMAN PAPILLOMAVIRUS 16 GENOME FROM CERVICAL CANCER TISSUE [J] . 楚雍烈, 刘延娜, 袁育康, 药物分析学报：英文版 . 1998,第002期
2. Effects of human papillomavirus type-specific antisense oligonucleotides on the proliferation of cervical cancer cells containing papillomavirus type 16 [J] . 穆润华, 陈必良, 郑维国, 中国人民解放军军医大学学报：英文版 . 1999,第001期
3. HPLC ASSAY FOR INTRACELLULAR ACCUMULATION OF VERAPAMIL IN VER-RESISTANT HUMAN LEUKEMIC CELL SUSLINES AND THEIR PARENTAL CELL LINES [J] . Xie Zuofu, Lin xiandong, Zhou Dongmei, 中国癌症研究（英文版） . 1998,第003期
4. Retraction: Investigation of cross‐contamination among human cell lines used in China by Mingzhen Wang, Meimei Yang, Yuehong Liu, Yaqing Huang, Fang Ye, Congyi Zheng and Chao Shen [J] . International Journal of Cancer =: Journal International du Cancer . 2018,第7期

机译：Retraction: Investigation of cross‐contamination among human cell lines used in China by Mingzhen Wang, Meimei Yang, Yuehong Liu, Yaqing Huang, Fang Ye, Congyi Zheng and Chao Shen
5. Retraction: Investigation of cross‐contamination among human cell lines used in China by Mingzhen Wang, Meimei Yang, Yuehong Liu, Yaqing Huang, Fang Ye, Congyi Zheng and Chao Shen [J] . International Journal of Cancer =: Journal International du Cancer . 2018,第7期

机译：Retraction: Investigation of cross‐contamination among human cell lines used in China by Mingzhen Wang, Meimei Yang, Yuehong Liu, Yaqing Huang, Fang Ye, Congyi Zheng and Chao Shen
6. The study and detection of human papillomavirus (HPV) genome in two cervical carcinoma cell lines by the use of hybridization techniques. [O] . 1990

机译：The study and detection of human papillomavirus (HpV) genome in two cervical carcinoma cell lines by the use of hybridization techniques.

An Exonuclease V–qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Lines and Tissues

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