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首页> 外文期刊>Current Protocols in Microbiology >A Mouse Model of Sublethal Leptospirosis: Protocols for Infection with Leptospira Through Natural Transmission Routes, for Monitoring Clinical and Molecular Scores of Disease, and for Evaluation of the Host Immune Response
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A Mouse Model of Sublethal Leptospirosis: Protocols for Infection with Leptospira Through Natural Transmission Routes, for Monitoring Clinical and Molecular Scores of Disease, and for Evaluation of the Host Immune Response

机译：亚致死的钩端螺旋体病的小鼠模型:协议为钩端螺旋体感染自然传播途径,为监测疾病的临床和分子分数,对宿主免疫反应的评价

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摘要

Leptospirosis is a zoonotic disease caused by pathogenic Leptospira species that are maintained in sylvatic and domestic environments by transmission among rodents and other carriers. Humans become infected after contact of breached skin or mucosa with contaminated water or soil. Understanding persistent or sublethal infection in a host is critical for controlling human risk of exposure to pathogenic Leptospira. Animal models that recapitulate disease progression after infection via natural transmission routes are more appropriate for validation of vaccines and therapeutics. Furthermore, the ability to measure shedding of live Leptospira in urine of reservoir and carrier hosts can be used to develop new diagnostic assays and sensors to evaluate human risk of exposure. We developed inbred mouse models of Leptospirosis, that bypass survival as a criterion, in which we can analyze both pathogen and host factors affecting sublethal infection (<1 month), including shedding of Leptospira in urine. Mice are infected with pathogenic Leptospira using a physiologic route, and the clinical, histological, and molecular scores of disease are measured. Furthermore, the host immune response to Leptospira is evaluated. This mouse model also provides a tool in which to test fundamental hypotheses related to host‐pathogen interactions and the immune mechanisms engaged in protective and pathogenic immune responses.
机译：钩端螺旋体病是一种人畜共患疾病所致致病性钩端螺旋体物种森林型和国内环境中啮齿动物和其他运营商之间传播。人类接触后被感染的突破皮肤或粘膜与受污染的水或土壤。理解持久或尚不致命的感染在主机控制人类的风险是至关重要的暴露在致病性钩端螺旋体。模型概括疾病进展感染后通过自然传播路线更适合的验证疫苗和治疗。测量的钩端螺旋体的尿液水库和载体主机可以用来开发新的诊断化验和传感器评估人类暴露的风险。纯系小鼠模型的钩端螺旋体病,绕过生存是一个标准,我们可以分析病原体和宿主因素影响(< 1个月),包括尚不致命的感染脱落的钩端螺旋体尿液。使用一个致病性钩端螺旋体感染生理途径,临床组织学和分子分数的疾病测量。钩端螺旋体是评估。提供了一个工具来测试基础假设与宿主病原体相互作用和免疫机制参与保护和致病性免疫反应。

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