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首页> 外文期刊>Current Protocols in Microbiology >Infection and Propagation of AstrovirusVA1 in Cell Culture
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Infection and Propagation of AstrovirusVA1 in Cell Culture

机译：AstrovirusVA1在细胞的感染和传播文化

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摘要

Astrovirus VA1/HMO-C (VA1) is the representative genotype of mamastrovirus9, a species of the single-stranded, positive-sense RNA viral family,Astroviridae. Astroviruses have been traditionally considered pathogens of thegastrointestinal tract but they have been recently associated with neurologicaldiseases in humans, cattle, mink, sheep, and pigs. VA1 is the astrovirus genotypemost commonly identified from human cases of meningoencephalitis andhas been recently propagated in cell culture. VA1 can now be used as a modelsystem to study pathogenesis of the neurological diseases associated with astrovirusinfection. In this article, we describe two fundamental assays to quantifyreplication and propagation of VA1, a quantitative reverse transcription-PCR(qRT-PCR) to measure viral RNA and a 50% tissue culture infectious dose(TCID50) assay to measure infectious viral particles.
机译：Astrovirus VA1 / HMO-C (VA1)是代表mamastrovirus9基因型,一个物种的单链RNA病毒的积极意义家庭,Astroviridae。传统上认为病原体thegastrointestinal束但他们最近与neurologicaldiseases有关人类、牛、貂、羊和猪。astrovirus genotypemost普遍确认的人类脑膜脑炎病例,并且最近在细胞培养中传播。被用作modelsystem研究发病机理与相关的神经系统疾病astrovirusinfection。两个基本的化验quantifyreplication和VA1的传播定量逆转一(存在)来衡量病毒RNA和50%的组织培养感染剂量(TCID50)试验测量感染性病毒颗粒。

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