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首页> 外文期刊>Current Protocols in Microbiology >Encephalitozoon: Tissue Culture,Cryopreservation, and Murine Infection
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Encephalitozoon: Tissue Culture,Cryopreservation, and Murine Infection

机译：Encephalitozoon:组织文化,低温贮藏,和小鼠感染

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摘要

Microsporidia are eukaryotic unicellular parasites that have been studied formore than 150 years. They are found throughout the world and are capableof infecting various invertebrate and vertebrate hosts. They can causedisease in both immune-compromised and immune-competent humans. Inimmune-compromised individuals, infections can be severe and often fatal.Microsporidia possess a unique, highly specialized invasion mechanism thatinvolves a structure known as the polar tube as well as the spore wall. Duringspore germination, the polar tube rapidly discharges from the spore and deliverthe sporoplasm into the host cell. Spores are the only stage of microsporidiathat can survive outside of host cells. Since the first attempt to culture microsporidiain vitro in 1930s, their cultivation has served a critical role in thestudy and diagnosis of these parasites. In this chapter, we include methods onthe cultivation, isolation, and cryopreservation of Encephalitozoon cuniculi,which can infect humans and provides a useful model for other microsporidia.These methods can also be utilized for the culture of Encephalitozoon hellemor Encephalitozoon intestinalis.
机译：小孢子虫目是真核单细胞寄生虫150年多研究。他们发现整个世界对感染各种无脊椎动物脊椎动物宿主。职业和immune-competent人类。Inimmune-compromised个人、感染是严重的,常常是致命的。独一无二的,高度专业化的入侵机制thatinvolves结构被称为极地管以及孢子壁。发芽,极地管迅速排放从孢子和deliverthe孢子质进入宿主细胞。microsporidiathat能生存以外的主机细胞。microsporidiain体外在1930年代,他们的培养在这项研究中扮演一个关键角色,这些寄生虫的诊断。包括方法在培养、隔离和低温贮藏的Encephalitozooncuniculi,可感染人类并提供为其他小孢子虫目有用的模型。方法也可以用于文化Encephalitozoon hellemor Encephalitozoonintestinalis。

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