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首页> 外文期刊>Current Protocols in Microbiology >Quantitative Proteomic Profiling of Cryptococcus neoformans
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Quantitative Proteomic Profiling of Cryptococcus neoformans

机译：隐球菌的定量蛋白质组学分析neoformans

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摘要

Cryptococcus neoformans is an opportunistic human fungal pathogen commonly associated with infection in immunocompromised individuals (e.g., patients with HIV/AIDS). Important virulence determinants include the production of a polysaccharide capsule,melanin, and extracellular enzymes, as well as the ability to grow at 37°C. C. neoformans controls a plethora of host defense and evasion mechanisms to survive during infection and to proliferate within the host, causing meningoencephalitis and death. Traditionally, characterization of C. neoformans under different environmental conditions and stresses has relied on genetic and phenotypic analyses, as well as biochemical assays. However, advances in mass spectrometry instrumentation, sample preparationprotocols, and bioinformatic tools and databases promote comprehensive profiling of fungal cellular processes, secretion or protein release into the extracellular environment, and vesicle contents. Moreover, proteomics provides insight into regulatory mechanisms influencing signal transduction cascades and protein complexes or networks through profiling of post‐translational modifications and protein–protein interactions. Given the medical impact of C. neoformans infections and the recent emergence of antifungal‐resistant strains, defining proteins produced in response to unique environments provides an opportunity to uncover antivirulence strategies and alternative therapeutic options to combat infection. Here, we describe culturing and sample preparation of C. neoformans and outline protocols for comprehensively profiling changes in protein abundance within the cellular proteome and secretome.
机译：新型隐球菌是一种投机取巧的人真菌病原体通常联系在一起感染免疫功能不全的个人(例如,艾滋病毒/艾滋病患者)。决定因素包括生产多糖胶囊、黑色素和细胞外酶,以及生长在37°C的能力。c . neoformans控制过多的宿主防御和逃避机制中生存在宿主感染和扩散,引起脑膜脑炎和死亡。新生隐球菌。传统上,表征在不同的环境条件强调依靠遗传和表型分析、生化分析。质谱分析仪器的进步,样本preparationprotocols和生物信息学促进全面的工具和数据库真菌细胞过程的剖析,分泌或蛋白质释放到细胞外环境和囊泡的内容。蛋白质组学提供了洞察监管影响信号转导机制瀑布和蛋白质复合物或网络通过分析文章的转化修改和蛋白质相互作用。新生隐球菌。考虑到医学的影响感染和最近出现的抗真菌检测耐药菌株,定义蛋白质在独特的环境发现antivirulence提供了一个机会策略和替代治疗选项对抗感染。新生隐球菌和样品制备的轮廓用于全面分析变化的协议细胞内的蛋白质丰富蛋白质组检查参与组成分泌腺和。

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