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首页> 外文期刊>Current Protocols in Microbiology >Shigella Pathogenesis Modelingwith Tissue Culture Assays
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Shigella Pathogenesis Modelingwith Tissue Culture Assays

机译：志贺氏杆菌发病Modelingwith组织文化化验

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摘要

Shigella is an enteroinvasive human pathogen that infects the colonic epitheliumand causes Shigellosis, an infectious diarrheal disease. There is no vaccine forthe prevention or treatment of Shigellosis and antibiotic-resistant strains ofShigella are increasing, emphasizing the need for a deeper understanding ofShigella pathogenesis in order to design effective antimicrobial therapies. Smallanimal models do not recapitulate Shigellosis, therefore tissue-cultured cellshave served as model systems to study Shigella pathogenesis. Here, protocolsto enumerate Shigella invasion, cell-cell spread, and plaque formation in thetissue-cultured cell lines Henle-407 and CoN-841 are described. Additionally,a new method to study Shigella invasion in primary intestinal enteroids isdescribed. These protocols can be used to examine different aspects of Shigellavirulence.
机译：志贺氏杆菌是一种enteroinvasive人类病原体感染的结肠epitheliumand原因志贺氏菌病、传染性腹泻病。为预防或没有疫苗志贺氏菌病的治疗和抗生素耐药菌株ofShigella正在增加,强调ofShigella需要更深层次的理解发病机制为了设计有效抗菌治疗。不是概括志贺氏菌病,因此组织培养cellshave作为模型系统志贺氏杆菌发病机理进行研究。列举志贺氏杆菌入侵,信息传播,在thetissue-cultured细胞和斑块的形成亨利- 407和反面- 841行。此外,一个新的方法来研究志贺氏杆菌在主要的肠肠状的入侵isdescribed。检查Shigellavirulence的不同方面。

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