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首页> 外文期刊>Current Protocols in Microbiology >Salmonella Typhimurium Infection ofHuman Monocyte-Derived Macrophages
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Salmonella Typhimurium Infection ofHuman Monocyte-Derived Macrophages

机译：鼠伤寒沙门氏菌感染人类Monocyte-Derived巨噬细胞

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摘要

The successful infection of macrophages by non-typhoidal serovars ofSalmonella enterica is likely essential to the establishment of the systemicdisease they sometimes cause in susceptible human populations. However, theinteractions between Salmonella and human macrophages are not widely studied,with mouse macrophages being a much more common model system.Fundamental differences between mouse and human macrophages make thisless than ideal. Additionally, the inability of human macrophage-like cell linesto replicate some properties of primary macrophages makes the use of primarycells desirable. Here we present protocols to study the infection of humanmonocyte-derived macrophages with Salmonella Typhimurium. These includea method for differentiating monocyte-derived macrophages in vitro and protocolsfor infecting them with Salmonella Typhimurium, as well as assays tomeasure the extent of infection, replication, and death. These protocols areuseful for the investigation of both bacterial and host factors that determine theoutcome of infection.
机译：成功的感染的巨噬细胞non-typhoidal型ofSalmonella血清可能的建立至关重要systemicdisease他们有时引起的易感人群。沙门氏菌和人类之间的互动巨噬细胞还没有广泛研究,但与鼠标巨噬细胞作为一个更为常见的模型系统。人类巨噬细胞使thisless比理想。此外,人类的能力macrophage-like细胞linesto复制一些初级巨噬细胞使使用的属性的primarycells可取的。协议的感染humanmonocyte-derived巨噬细胞与沙门氏菌沙门氏菌感染。区分monocyte-derived巨噬细胞在体外和protocolsfor感染他们鼠伤寒沙门氏菌,以及化验程序的感染、复制、和死亡。细菌和宿主因素的调查决定theoutcome感染。

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