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首页> 外文期刊>Current Protocols in Microbiology >Human Intestinal Enteroids for the Studyof Bacterial Adherence, Invasion, andTranslocation
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Human Intestinal Enteroids for the Studyof Bacterial Adherence, Invasion, andTranslocation

机译：人类肠道Studyof肠状的细菌粘附,入侵,andTranslocation

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摘要

Adherence, invasion, and translocation to and through the intestinal epitheliumare important drivers of disease for many enteric bacteria. However, mostwork has been limited to transformed intestinal cell lines or murine modelsthat often do not faithfully recapitulate key elements associated with humandisease. The recent technological advances in organotypic tissue and cell cultureare providing unparalleled access to systems with human physiology andcomplexity. Human intestinal enteroids (HIEs), derived from patient biopsyor surgical specimens of intestinal tissues, are organotypic cultures now beingadapted to the study of enteric infections. HIEs are comprised of the dominantcell types of the human gastrointestinal epithelium, can be grown in two- orthree-dimensional structures, form a crypt–villus axis with defined apical andbasolateral compartments, and undergo physiologic responses to many differentstimuli. Here, we describe a series of protocols that encompass the use ofhuman enteroids for the measurement of the adherence, invasion, and translocationof E. coli to and through the intestinal epithelium. We also outline thesteps needed to grow and prepare enteroids for this purpose and highlight somecommon problems to troubleshoot.
机译：坚持,入侵和易位通过肠道epitheliumare重要司机很多肠疾病的细菌。然而,mostwork转换是有限的肠细胞系或小鼠modelsthat经常不忠实地概括关键要素与humandisease有关。在organotypic组织和技术进步细胞cultureare提供无与伦比的访问权系统与人类生理和复杂性。人类的肠肠状的(麻疹),来自病人biopsyor手术标本的肠道组织,现在organotypic文化beingadapted肠道感染的研究。麻疹是由dominantcell类型的人类胃肠道上皮细胞生长在两orthree-dimensional结构,形成一个crypt-villus轴与定义的顶端andbasolateral隔间,并接受许多differentstimuli生理反应。在这里,我们描述的一系列协议包含的使用人类肠状的测量的依从性、侵略和并通过translocationof大肠杆菌肠道上皮细胞。需要成长和肠状的准备目的和突出somecommon问题进行故障排除。

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