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首页> 外文期刊>Current Protocols in Microbiology >Use of Axenic Culture Tools to StudyCoxiella burnetii
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Use of Axenic Culture Tools to StudyCoxiella burnetii

机译：使用无菌培养StudyCoxiella的工具burnetii

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摘要

Coxiella burnetii is a highly infectious obligate intracellular bacterium andthe etiological agent of the zoonosis Query (Q) fever. This Gram-negativegamma-proteobacterium has adapted to replicate within a specialized compartmentin mammalian phagocytic cells, known as the Coxiella-containingvacuole (CCV). Knowledge of critical characteristics of the CCV microenvironment(e.g., luminal pH), analysis of the C. burnetii genome sequence, andstrategicmetabolic profiling have provided the basis for determining the physicochemicaland nutritional conditions necessary to support axenic replicationof C. burnetii. In this unit, the media currently utilized for axenic culture ofC. burnetii are described, with emphasis on application. To aid in experimentalreproducibility and interpretation of results, considerations and limitations arediscussed. Lastly, expected results for C. burnetii axenic growth under controlconditions are provided as a reference.
机译：伯纳特氏立克次氏体是一种高度传染性预留细胞内的细菌和病因代理人人畜共患病的查询(Q)发热。Gram-negativegamma-proteobacterium已经适应了在专业compartmentin复制哺乳动物的吞噬细胞,被称为Coxiella-containingvacuole(闭路)。闭路的关键特征微环境(如贝纳特氏立克次c基因组序列,andstrategicmetabolic分析提供了确定physicochemicaland的基础营养条件的必要支持贝纳特氏立克次无菌replicationof c。媒体目前用于无菌培养离岸金融中心。应用程序。experimentalreproducibility和解释的结果,注意事项和限制了。burnetii无菌条件对照下成长提供作为参考。

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