Isolation and Whole-Genome Sequencingof Environmental Campylobacter

Brittni R. Kelley; J. Christopher Ellis; Doug HyattDan JacobsonJeremiah Johnson

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Isolation and Whole-Genome Sequencingof Environmental Campylobacter

机译：隔离和全基因组Sequencingof环境弯曲杆菌

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As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacterhas a significant impact on human health. In the developed world, mostcampylobacteriosis cases are attributed to the consumption of undercooked,contaminated poultry; however, it has been shown that Campylobacter can betransmitted to humans through contaminated water and other types of food,including beef and milk. As such, high-resolution microbial source-tracking isessential for health department officials to determine the source(s) of Campylobacteroutbreaks. For these reasons, this protocol provides the techniquesneeded for isolation of Campylobacter from agricultural and environmentalsources, as well as human clinical specimens. Additionally, we describe asimple method for preparing high-quality genomic DNA that can be used forwhole-genome sequencing and downstream bioinformatics analyses of Campylobactergenotypes.

机译：作为一个bacterial-derived的主要原因全球肠胃炎,Campylobacterhas对人类健康产生重大影响。发达国家,mostcampylobacteriosis病例归因于的消费未煮熟的家禽污染;表明,弯曲杆菌可以betransmitted通过受污染的水和其他人类类型的食品,包括牛肉和牛奶。高分辨率的微生物源跟踪新卫生部门官员确定的来源(s)Campylobacteroutbreaks。协议提供了techniquesneeded从农业和隔离的弯曲杆菌environmentalsources以及人类临床标本。准备高质量的基因组DNA的方法可以使用forwhole-genome测序下游的生物信息学分析Campylobactergenotypes。

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来源
《Current Protocols in Microbiology》 |2018年第1期|s64/1-e64/16|共16页
作者
Brittni R. Kelley; J. Christopher Ellis; Doug HyattDan JacobsonJeremiah Johnson;
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作者单位

Oak Ridge National Laboratory, Oak Ridge, Tennessee;

Department of Microbiology, University of Tennessee, Knoxville, Tennessee;

Bredesen Center, University of Tennessee, Knoxville, Tennessee;

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原文格式 PDF
正文语种英语
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关键词
human health; whole genome sequencing; Campylobacterwater pollution;

机译：人类健康;全基因组测序;Campylobacterwater污染;

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Isolation and Whole-Genome Sequencingof Environmental Campylobacter

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