Human Papillomavirus Integration:Analysis by Molecular Combing andFiber-FISH

Catherine J. Redmond; Haiqing Fu; Mirit I. AladjemAlison A. McBride

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Human Papillomavirus Integration:Analysis by Molecular Combing andFiber-FISH

机译：人类乳头状瘤病毒整合:分析分子结合andFiber-FISH

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Human papillomaviruses (HPVs) are frequently integrated in HPV-associatedcancers. HPV genomes can be integrated in three patterns: A single integratedHPV genome (type I), multiple, tandemly integrated HPV genomes (type II),and multiple, tandemly integrated HPV genomes interspersed with host DNA(type III). Analysis of the organization of type II and type III integration sites iscomplicated by their repetitive nature, as sequences of individual repeats are dif-ficult to distinguish from each other. This article presents a method for directlyvisualizing HPV integration sites using molecular combing combined withfluorescent in situ hybridization, also known as fiber-FISH. In this technique,genomic DNA is stretched across a glass coverslip and individual integratedHPV sequences are detected and directly visualized by in situ hybridizationwith a resolution of 1 kb. Fiber-FISH allows comprehensive characterizationof the genomic organization of HPV integration sites containing type II andtype III integration.

机译：人类乳头状瘤病毒(人类乳头瘤病毒)经常在HPV-associatedcancers集成。可以集成在三个模式:一个单身吗integratedHPV基因组(I型)、多个衔接着集成HPV基因组(II型),多个衔接着集成HPV基因组穿插宿主DNA (III型)。该组织的分析类型II和III型集成网站iscomplicated重复性质,单个重复序列佩区分彼此。提出了一种directlyvisualizing人乳头状瘤病毒的方法集成网站使用分子结合withfluorescent原位杂交相结合,也称为fiber-FISH。技术,基因组DNA是横跨一个玻璃盖玻片和个人integratedHPV序列直接检测并通过原位可视化hybridizationwith分辨率为1 kb。Fiber-FISH允许全面characterizationof基因的组织人乳头状瘤病毒包含II型andtype集成网站三世集成。

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来源
《Current Protocols in Microbiology》 |2018年第1期|e61/1-e61/16|共16页
作者
Catherine J. Redmond; Haiqing Fu; Mirit I. AladjemAlison A. McBride;
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作者单位

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, Bethesda, Maryland;

Center for Cancer Research, National Cancer Institute, National Institutes of Health,Bethesda, Maryland;

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正文语种英语
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关键词
Human Papilloma Virus; Genomics; combedpapillomavirusesGenome;

机译：人类乳头状瘤病毒;基因;combedpapillomavirusesGenome;

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Human Papillomavirus Integration:Analysis by Molecular Combing andFiber-FISH

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