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首页> 外文期刊>Deutsche Zeitschrift fur Sportmedizin >Belastungsinduzierte DNA-Effekte und Hämoxygenase-Expression in Leukozyten
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Belastungsinduzierte DNA-Effekte und Hämoxygenase-Expression in Leukozyten

机译：Belastungsinduzierte DNA-Effekte和

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We studied the occurance of DNA damage in leucocytes and separated mononuclear cells (MN) of 10 subjectes after a half marathon run (HM) using the single cell gelelectropho-resis (Comet-) assay. Additionally the cyto-plasmic expression of the antioxidant stress-protein heme oxygenase-1 (HO-1) in lympho-(L), mono- (M) and granulocytes (G) was analysed by flow cytometry. Total leucocytes exhibited a greater frequency of DNA damaged cells (33%) 24 h after HM compared to separated mononuclear cells (15%), indicating a greater extent of DNA effects in G. Single MN showed a pronounced extent of DNA damage and the frequency of DNA effects in MN 24 h after HM was related to changes in plasma TNFa directly after exercise. Cytoplasmic HO-1 rose in L, G and M, but G and M exhibited a more pronounced expression 0, 3 and 24 h after HM. Differences in DNA damage and the expression of HO-1 between the leucocyte populations after HM let us suggest, that M and G are critical target cells susceptible to exercise induced oxidative stress.
机译：我们研究DNA损伤的出现白细胞和单核细胞分离(MN)半程马拉松跑后的10个主题(HM)使用单细胞gelelectropho-resis(彗星)测定。抗氧化剂stress-protein血红素的表达oxygenase-1 HO-1 lympho - (L), mono - (M)和粒细胞(G)被流式细胞术分析。总白细胞表现出更大的频率DNA受损的细胞(33%)后24 h HM相比分离单核细胞(15%),表明更大程度上在g .单一MN DNA的影响显示明显的DNA损伤的程度DNA的频率效应在MN HM后24 h等离子体后直接TNFa相关变化锻炼。但G和M表现出更明显表达0、3、HM后24小时。DNA损伤和HO-1的表达嗯让我们建议后白细胞的数量,M和G靶细胞至关重要容易锻炼诱导氧化应激。

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