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首页> 外文期刊>Vegetos: an international journal of plant research >Estimation of total phenolic content, total flavonoid content and antioxidant capacities of five medicinal plants from Nepal
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Estimation of total phenolic content, total flavonoid content and antioxidant capacities of five medicinal plants from Nepal

机译：估计总酚含量、总类黄酮含量和抗氧化能力五个来自尼泊尔的药用植物

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Oxidative stress imposed by increased concentration of free radicals, particularly, Reactive Oxygen Species (ROS) has been known to contribute to the initiation and progression of several diseases. Plant derived antioxidants can counteract oxidative stress and are capable of reducing the risk of developing these diseases. In the present study, the methanolic extracts of five medicinal plants were tested for their antioxidant capacities using different antioxidant assays. The total flavonoid and phenolic contents of the extracts were determined and a correlation was established between antioxidant capacities and total phenol/flavonoid content. Among the tested extracts, the highest amount of total phenolic and total flavonoid content was found in methanol extract of KUPS_1(,Rheum australe) with TPC value of 249.58 ± 7.73 Gallic acid equivalent mug/mg and TFC value of 480.84 ± 8.81 Rutin equivalent mug/mg. All the samples showed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and Ferric Reducing Activity Potential (FRAP) antioxidant activities at different levels. Highest DPPH radical scavenging was shown by KUPS_1(,Rheum australe) (IC_(50) = 3.47 ± 0.09), ABTS radical were most efficiently quenched by KUPS_5(Bergenia ciliata) (IC_(50) = 3.82 ± 0.63) and the highest reducing potential was shown by KUPS_2(Nirbikhi) (FRAP = 61.76 ± 2.29 equivalent mug Fe~(2+)/ml). A significant correlation was found between the antioxidant activity of extractsand their total phenolic and total flavonoid contents.
机译：氧化应激的增加自由基的浓度,特别是,活性氧(ROS)已经知道的发生和发展做出贡献一些疾病。对抗氧化应激和有能力降低罹患这些疾病的风险。在目前的研究中,methanolic提取的五个的药用植物进行了测试使用不同的抗氧化能力抗氧化试验。酚类内容的提取测定之间建立了联系抗氧化能力和总酚类黄酮内容。总酚和总类黄酮内容被发现在甲醇提取的KUPS_1(,大黄精华)TPC值为249.58±7.73没食子酸当量杯/ mg和交通值为480.84±8.81芦丁等效杯/毫克。所有的样品显示2, 2-azino-bis (3-ethylbenzothiazoline-6-sulphonic酸)(abt)和铁减少活动潜在(收紧)抗氧化活动不同的级别。KUPS_1所示(,大黄精华)(IC_ (50) =3.47±0.09),abt激进是最有效的由KUPS_5淬火(Bergenia ciliata) (IC_ (50) =3.82±0.63)和减少最高的潜力显示了KUPS_2 (Nirbikhi)(收紧= 61.76±吗2.29等效杯子Fe ~(2 +) /毫升)。抗氧化剂之间的相关性被发现extractsand他们的总酚和活动总类黄酮含量。

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