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首页> 外文期刊>Vegetos: an international journal of plant research >Bio-nematicidal activities by culture filtrate of Bacillus subtilis HussainT-AMU: new promising biosurfactant bioagent for the management of Root Galling caused by Meloidogyne incognita
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Bio-nematicidal activities by culture filtrate of Bacillus subtilis HussainT-AMU: new promising biosurfactant bioagent for the management of Root Galling caused by Meloidogyne incognita

机译：Bio-nematicidal活动文化的滤液枯草芽孢杆菌HussainT-AMU:新的承诺生物表面活性剂bioagent管理根磨损造成的有隐姓埋名的女人

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摘要

Root galling caused by Meloidogyne incognita causes dramatic yield reduction in a wide range of crops, especially vegetables, at most 15-25%, however, in severe cases it can reach upto 75%. A new biosurfactant bacteria characterized through 16S rRNA gene sequencing and biochemically as Bacillus subtilis HussainT-AMU that was isolated from oil contaminated soil from Odisha coat line near Paradip port exhibiting significant biosurfactant activity in all screening assays. Most of the in vitro experiments were carried out at room temperature. Exposure of fresh culture filtrate from new bioagent to M. incognita caused substantial mortality of the second stage juvenile (J2) and significantly reduced egg hatchability. 100% of culture filtrate collected from B. subtilis HussainT-AMU caused J2 mortality and reduced hatching (p < 0.05). Hatching was defective by 97-99%. More than 90% J2 mortality occurred after 24 h of exposure in 100%. Significant reduction (58.79%) in number of galls/plant in tomato rootsin combined application leading to reduction in root galling was recorded. This novel biosurfactant bacterial strain seems to be a safe and effective bio-nematicide potential that needs to be explored more both at field study as well genomic level.
机译：根有incognita造成的磨损导致一系列戏剧性的减少产量作物,特别是蔬菜,最多15 - 25%,然而,在严重的情况下,可以达到高达75%。新的生物表面活性剂细菌特征16 s rRNA基因测序和生化反应枯草芽孢杆菌HussainT-AMU孤立从石油污染土壤Odisha外套名港附近表现出显著的生物表面活性剂的活动在所有筛选化验。大部分的体外实验进行在室温下。滤液从新的bioagent m .隐姓埋名的女人大量的死亡率的第二阶段少年(J2)和显著降低鸡蛋孵化率。从枯草芽孢杆菌HussainT-AMU J2死亡率造成的并降低孵化(p < 0.05)。有缺陷的97 - 99%。经过24小时的接触发生在100%。数量显著减少(58.79%)茄rootsin擦伤/植物的总和应用程序导致根减少磨损被记录。压力似乎是一种安全、有效bio-nematicide潜在的需要探索更多的基因组在野外研究的水平。

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