• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vegetos: an international journal of plant research >Characterization and biocontrol measures of Pseudomonas syringae pv. syringae associated with citrus blast disease
【24h】

Characterization and biocontrol measures of Pseudomonas syringae pv. syringae associated with citrus blast disease

机译：表征和生物防除措施丁香假单胞菌。柑橘爆炸疾病

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study was conducted to identify the two Pseudomonas syringae pv. syringae strains attained from the citrus blast infected tissues and also to find out the antimicrobial potentiality of plant extracts and antagonistic bacteria Bacillus spp.both under in vitro and in vivo conditions. Based on the microscopic observation and biochemical features including colouring, magnitude, size, and form of colonies displayed on LB medium, we speculate that ps17a and ps18a strains were similar to P. syringae pv syringae. For molecular identification, 16S rRNA and multi-locus sequence analysis (MLSA) with two housekeeping gene (rpoD and gyrB) sequences indicated an identical pattern to the corresponding sequences of other P. syringae pv. syringae strains which confirmed that a genetically homologous species of P. syringae pv. syringae was responsible for bacterial blast disease of citrus cultivars. Later, PCR amplification using two primer pairs (B1 and B2) yielded 752-bp fragments to confirm that syrBgene present on both isolated P. syringae pv syringae and reference strains (Pss16) can synthesize syringomycin, a potent toxin for plant pathogenicity induction. Based on the pathogenicity tests, two strains were similar in terms of virulence activity,however, were not analogous thus, having the same level of severity effect to induce bacterial blast. Moreover, antibiotic sensitivity assay showed disproportionate result for both strains which resemble susceptible strains determination. From the in vitro investigation of six plant extracts and three Bacillus isolates against ps17a and ps18a, the ethanolic extract of Allium sativum, Moringa oleifera, and Azadirachta indica showed the highest antibacterial activity against two isolated bacterial strains up to inhibition zone 23.4, 24.4 and 18.2 mm in diameter, respectively while Bacillus isolates proved to be better natural antimicrobial agents against the disease. Finally, based on in vivo experiment, the most effective plant extracts and antagonistic isolates BS5 exhibited significant necrosis growth reduction after 10 weeks of inoculation by ps17a up to 67.48 and 52.88% as compared to 65.42 and 55.3% by ps18a, respectively. Thus, six selected ethanolic plant extracts and three isolates of Bacillusspp. have a broad-spectrum antimicrobial activity which can be used as effective biocontrol agent.
机译：本研究进行了识别两种丁香假单胞菌。达到从柑橘爆炸感染组织并找出抗菌素潜力的植物提取物和敌对细菌芽孢杆菌在体外和spp.both体内条件。包括观察和生化特性色彩、大小、大小和形式的殖民地显示在LB培养基,我们推测ps17a丁香和ps18a菌株是类似于p两。和多位点序列分析(二)有两个管家基因(rpoD和gyrB)序列表示一个相同的模式相应的其他丁香p序列。证实,两株丁香p基因同源物种。两负责细菌的爆炸柑橘品种的疾病。使用两对引物扩增(B1和B2)产生了752 - bp确认syrBgene碎片目前在两个孤立的丁香p两和参考菌株(Pss16)可以合成syringomycin,植物的毒素致病性归纳。致病性试验,两种都是相似的然而,毒性方面的活动类似这样,拥有相同级别的严重性影响诱导细菌爆炸。抗生素敏感性试验显示不成比例的结果这两株像敏感菌株的决心。六个植物提取物的体外研究和三个芽孢杆菌对ps17a和隔离ps18a, ethanolic提取大蒜,辣木属鉴定,Azadirachta indica显示对两个最高的抗菌活性分离菌株抑菌圈分别为23.4、24.4和18.2毫米直径而芽孢杆菌分离株被证明是更好的天然抗菌药物对疾病。最后,基于体内实验,最多有效的植物提取物和敌对隔离BS5表现出明显的坏死增长减少10周后接种ps17a 65.42比67.48和52.88%由ps18a和55.3%,分别。选择乙醇植物提取物及其三个Bacillusspp隔离。可作为抗菌活性有效的生物防治剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号