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首页> 外文期刊>Vegetos: an international journal of plant research >Foliar micro-morphology: a promising tool to improve survival percentage of tissue culture raised plantlets with special reference to in vitro propagation of Vitexnegundo L.
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Foliar micro-morphology: a promising tool to improve survival percentage of tissue culture raised plantlets with special reference to in vitro propagation of Vitexnegundo L.

机译：叶面micro-morphology:一个有前途的工具改善生存的组织文化提高植株与特殊的参考体外传播Vitexnegundo L。

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The in vitro induced anomalies during micropropagation affect acclimatization process of the regenerants, which influence the survival success of tissue culture raised plantlets. The comparative foliar micro-morphological analysis of in vitro developed; ex vitro adapted and field transferred plantlets could help in understanding the acclimatization of micropropagated plants. An enhanced shoot regeneration system for Vitex negundo L. was developed through axillary shoot regeneration and ex vitro rooting methods in present study. Murashige and Skoog’s medium (MS) incorporated with 1.5 mg L-1 6-benzylaminopurine (BAP) was recorded optimum for shoot regeneration from nodal segments. The shoots were further amplified (34.5 ± 0.21 shoots per culture vessel with 11.9 ± 0.10 cm average length) on full strength MS medium containing 0.5 mg L-1 BAP and 0.25 mg L-1 indole-3 acetic acid (IAA). The in vitro regenerated shoots were rooted under laboratory conditions (on half strength of MS medium incorporatedwith 1.0 mg L-1 IBA) as well as in greenhouse (ex vitro) when the shoots were treated with 300 mg L-1 IBA for 5 min. The hardened plantlets were successfully transferred to the field. The micro-morphological changes were observed in stomatal structure, index, veins and trichome density on the leaves developed in each stage of propagation of V. negundo. In vitro induced micro-morphological anomalies reduced stability of plant health and survival percentage which ultimately lead to the reduction in numberof plantlets. The keen foliar micro-morphological analysis at subsequent stages assisted in developing plantlets with 100% survival success in V. negundo.
机译：在体外诱导异常微体繁殖影响驯化过程再生剂的影响生存成功的组织培养植株。比较叶面微观分析体外发达;转化植株可以帮助理解micropropagated驯化的植物。牡荆增强射击再生系统牡l .通过腋窝开枪了再生和前男友体外方法,加油本研究。包含l - 1 6-benzylaminopurine 1.5毫克(BAP)记录拍摄再生的最佳对策从节段。放大(34.5±0.21每个培养皿芽平均11.9±0.10厘米长)中含有0.5毫克l - 1 BAP和女士的力量0.25毫克l - 1吲哚3乙酸(IAA)。体外再生芽根下实验室条件下(一半女士的力量媒介incorporatedwith 1.0毫克l - 1 IBA)温室(体外)交货时拍摄接受300毫克l - 1 IBA对5分钟。硬化的植株被成功转移这个领域。被观察到气孔结构、索引、静脉吗和毛状体密度在树叶中开发每个阶段诉牡的传播。诱导微观异常减少稳定的植物健康和生存百分比这最终导致在减少吗大量的植株。微观分析后续阶段协助发展与100%的植株生存在诉牡的成功。

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