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首页> 外文期刊>Vegetos: an international journal of plant research >Isolation and Identification of antifungal metabolite producing endophytic Bacillus subtilis (S17) and its in vitro effect on Colletotrichum falcatum causing red rot in sugarcane
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Isolation and Identification of antifungal metabolite producing endophytic Bacillus subtilis (S17) and its in vitro effect on Colletotrichum falcatum causing red rot in sugarcane

机译：抗真菌的分离和鉴定代谢物产生内生枯草芽孢杆菌(肌力)及其对炭疽菌体外的影响falcatum导致甘蔗红腐烂

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摘要

Red rot, a threatening disease of sugarcane caused by fungal pathogen Colletotrichum falcatum Went adversely affect the yield and quality of crop. Therefore, it is important to explore such methods of protection which are organically safe especially with the use of bioagents. Currently, usage of endophytic bacteria as biocontrol agent have been focussed in the field of biological control and enhancement of crop productivity. Hence, this study was centred on endophytic bacteria Bacillus subtilis isolate S17 (identified by 16S rRNA analysis) isolated from the sugarcane stalk tissues. The in vitro study indicated that isolate S17 inhibited the mycelial growth of C. falcatum by 76.22 ± 2.34% in dual-culture antagonism assay. Scanning electron microscopy (SEM) revealed the distortion and destruction in the hyphae of the fungal pathogen by isolate S17. Further investigations proved that the hyphal disorientation was by the secretion of various enzymes (chitinase, p-1,3 glucanase, protease, pectinase, and amylase), inhibitory substances viz., siderophore, ammonia, hydrogen cyanide (HCN) and other volatile as well as diffusible metabolites. The volatile and diffusible metabolites exhibited strong inhibition (74.57 ± 1.45% and 81.86 ± 2.36%) against C.falcatum, respectively. Similarly, cell-free culture supernatant that contains extracellular metabolites exhibited mycelial inhibition (70.52 ± 0.95%) against C. falcatum. Thus, the present finding revealed that different metabolites as well as variousexo-enzymes produced by B. subtilis (S17) inhibit red rot pathogen besides mechanism of the antagonism.
机译：红色腐烂,威胁疾病引起的甘蔗由真菌病原体刺盘孢属falcatum去了影响作物的产量和品质。因此,它是重要的去探索有机安全的保护方法尤其是在自然界的使用。使用的内生细菌作为生物防治剂一直专注领域的生物控制和提高作物生产力。因此,本研究主要集中在植物内生枯草芽孢杆菌细菌隔离肌力(被16 s rRNA分析)隔绝甘蔗茎的组织。表明,隔离肌力菌丝的抑制c . falcatum 76.22±2.34%的增长双文明对抗试验。显微镜(SEM)揭示了失真破坏菌丝的真菌病原体通过隔离肌力。的菌丝的迷失方向的分泌的各种酶(几丁质酶p - 1 3葡聚糖酶、蛋白酶、果胶酶和淀粉酶),即抑制性物质,含铁细胞、氨、氰化氢(HCN)和其他挥发性扩散性的代谢物。扩散性的代谢产物表现出强烈抑制(74.57±1.45%和81.86±2.36%)分别对C.falcatum。文化上层清液,其中包含游离细胞外代谢产物表现出菌丝体的抑制对c . falcatum(70.52±0.95%)。因此,目前的发现不同的显示代谢产物以及variousexo-enzymes由枯草芽孢杆菌(肌力)抑制红色腐烂除了机制对抗病原体。

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