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首页> 外文期刊>Vegetos: an international journal of plant research >Protein profiling of regenerative and non regenerative callus cultures of Glossonema varians: a rare, endemic and edible plant of Indian Thar Desert
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Protein profiling of regenerative and non regenerative callus cultures of Glossonema varians: a rare, endemic and edible plant of Indian Thar Desert

机译：再生和非蛋白质分析Glossonema再生愈伤组织文化瓦里安:一种罕见的、特有的、可食用的植物印度的塔尔沙漠

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摘要

Glossonema varians is a rare and endemic plant species of arid and semiarid region of Rajasthan, used as edible plant in Thar Desert. The plant is facing serious threat of extinction from its natural habitat due to overexploitation and slow propagation in natural conditions. Moreover, plant is known for its nutritional values, hence present manuscript is focused on protein profiling of G. varians. The internodal shoot explants were taken from in vitro grown cultures and inoculated on Murashige and Skoog’s (MS) medium augmented with 25 mg each of citric acid, arginine, adenine and 50 mg of ascorbic acid with different concentrations of PGRs for initiation of callus culture. A combination of 2, 4-D (2, 4-dichlorophenoxy acetic acid) and NAA (a-naphthalene acetic acid) was found to be suitable for optimum growth of callus. Regeneration of shoot buds was observed on MS medium augmented with a combination of BAP (6-benzylaminopurine), Kn (Kinetin), NAA and iP (N6-[2-isopentenyl] adenine). Comparative biochemical characterization of regenerative and non- regenerative callus has been done through in vitro quantification and protein profile has also been done by SDS-PAGE.
机译：Glossonema瓦里安是一个稀有和特有植物种干旱和半干旱地区的拉贾斯坦邦,在塔尔沙漠作为可食用的植物。面临严重的灭绝的威胁自然栖息地由于过度开采和缓慢在自然条件下传播。植物营养价值而闻名,因此现在的手稿是集中在蛋白质分析g·瓦里安。外植体来自体外生长的文化和接种Murashige和斯库(女士)中增强与25毫克的柠檬酸,精氨酸、腺嘌呤和50毫克的抗坏血酸不同浓度的pgr起始愈伤组织培养。4-dichlorophenoxy乙酸)和乙酰天冬氨酸(a-naphthalene醋酸)被发现最佳适合愈伤组织的生长。观察拍摄的再生芽女士中增强与BAP的组合(6-benzylaminopurine), Kn(激动素),NAA和iP(N6 - [2-isopentenyl]腺嘌呤)。再生和生化特征非再生愈伤组织已经通过体外量化和蛋白质也通过sds - page。

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