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首页> 外文期刊>Vegetos: an international journal of plant research >Effects of plant growth regulators on in vitro morphogenic response in Oldenlandia herbacea (L.) Roxb.
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Effects of plant growth regulators on in vitro morphogenic response in Oldenlandia herbacea (L.) Roxb.

机译：植物生长调节剂对体外的影响地貌成因的反应Oldenlandia herbacea (l)Roxb。

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摘要

Plant growth regulators (PGRs) may alter the morphogenic behavior of plant cells under in vitro conditions. Cytokinins and auxins were used to examine the response of mature nodal meristems of Oldenlandia herbacea (L.) Roxb. in this report. The 6-benzylaminopurine (BAP) alone at 1.5 mg L- 1 in Murashige and Skoog’s medium (MS) formed 5.0 ± 0.25 axillary shoots per explant but the combination of BAP, Kinetin (6-furfurylaminopurine) with indole-3-acetic acid (IAA) in the medium further proliferated the shoots exponentially (112.6 ± 0.68 shoots per explants per culture vessel). Auxins were used to induce roots from the cut ends of in vitro raised shoots via ex vitro method (in greenhouse). The highest percentage of ex vitro rooting (93%) with 11.5 ± 0.36 roots per shoot was achieved when shoots were pulse treated with 200 mg L- 1 indole-3-butyric acid (IBA) for 4 min. The regenerated plantlets were successfully acclimatized in the greenhouse and transferred to field with 89.6% survival rate. Thisis the foremost report on the use of plant growth regulators for effective in vitro regeneration via ex vitro rooting in O. herbacea. The developed system could be used as an alternative strategy to conserve this medicinal plant.
机译：植物生长调节剂(pgr)可能改变地貌成因的行为下的植物细胞体外条件。检查节点分生组织成熟的反应的Oldenlandia herbacea (l)报告。1.5毫克L - 1 Murashige和斯库的介质(女士)形成5.0±0.25腋窝芽外植体结合软面包卷,激动素(6-furfurylaminopurine) indole-3-acetic酸(IAA)中进一步扩散芽指数(112.6±0.68芽每个培养皿外植体)。体外诱导根切结束的芽通过例体外方法(温室)。例体外支持的比例最高(93%)11.5±0.36根每拍摄时实现芽是脉冲处理200毫克L - 1indole-3-butyric酸(IBA) 4分钟。再生植株被成功在温室和转移到服水土场存活率为89.6%。使用植物生长的最重要的报告监管机构有效的体外再生通过体外交货在o . herbacea加油。开发的系统可以作为一个替代选择策略来保护这一药用植物。

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