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首页> 外文期刊>Vegetos: an international journal of plant research >Characterization and association mapping for drought adaptation in Ethiopian sorghum (Sorghum bicolor (L.) Moench) germplasm
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Characterization and association mapping for drought adaptation in Ethiopian sorghum (Sorghum bicolor (L.) Moench) germplasm

机译：特征和关联映射干旱适应在埃塞俄比亚高粱(高粱二色的(l)

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Knowledge of drought related traits and their mechanisms of drought adaptation are the key component in selecting genotypes that withstand the effects of drought in sorghum. The objectives of this study were to assess genetic variability among sorghumgermplasm, to map QTL associated with root and shoot traits, and to identify tightly linked SSR markers. The experiment was carried out at Jimma University under greenhouse condition. One hundred thirty-six sorghum genotypes were characterized for twelve traits using a high throughput root phenotyping platform in a randomized complete block design with three replications. Among these genotypes, 108 of them were used for studying population structure and trait-marker association analysis using 39 SSR markers. The analysis of variance indicated that highly significant difference (P < 0.01) were observed among the genotypes for all the studied traits. The coefficients of correlation showed that there was a significant positive and negative association among different drought related traits. The first three principal components (PCs) with eigenvalues greater than one accounted for 56.4% of the total genotype variation, the remaining 43.60% accounted for the last nine principal components. All 136 genotype were grouped into four clusters whereby a different member within a cluster being assumed to be more closely related in terms of the trait under consideration with each other than that member in different clusters is. Moderate genotypic variation was exhibited for leaf area, shoot fresh weight, shoot dry weight, root to shoot ratio and root angle. Highest phenotypic variation was exhibited for leaf area, shoot fresh weight, shoot dry weight, root dry weight and root to shoot ratio. Broad sense heritability ranged from 19.35% for root length to 71.08% for shoot fresh weight and high heritability was recorded for shoot fresh weight (71.08%), leaf area (70.22%) and root angle (66.22%). High heritability combined with high genetic advance was observed for shoot fresh weight, root angle, and leaf area. The 108 genotypes were grouped into three distinct subgroups. The plots of LD (r2) showed a clear trend on linkage disequilibrium decay and based on trend line it is around 15-20 cM. A total of 25 significant marker-trait associations/QTLs (P < 0.05) were detected with 14 SSR markers.
机译：干旱相关的特征和他们的知识干旱的适应机制是关键承受的组件选择基因型高粱的干旱的影响。本研究评估遗传变异性以及sorghumgermplasm地图相关的QTL特征,生根,发芽和识别SSR标记紧密相连。在温室进行Jimma大学条件。基因型在12个性状特征使用一个高吞吐量根表现型的平台与三个随机完全区组设计复制。被用于研究人口结构和使用39 SSR trait-marker关联分析标记。高度显著性差异(P < 0.01)观察到的基因型的研究特征。有一个重大的积极的和负关联在不同的干旱相关的特征。组件与特征值大于(pc)一个占总额的56.4%的基因型变异,剩下的占43.60%最后9个主成分。分为四个集群,一个吗集群内不同成员的假定更密切相关的特征在考虑彼此在不同的集群成员。基因型的变化表现出叶面积,鲜重打击,干重、根射率和根角。变化表现出叶面积,开枪鲜重、干重,根干重和根拍摄比率。根的长度范围从19.35%到71.08%芽鲜重和高遗传为拍摄记录鲜重(71.08%)、叶区域(70.22%)和根角(66.22%)。遗传结合高遗传进展观察拍摄鲜重、根角,和叶面积。分成三个不同的子组。在连杆(r2)显示一个明确的趋势不均衡衰变和基于趋势线大约是15 - 20厘米。marker-trait协会/法(P < 0.05)与14个SSR标记检测。

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