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首页> 外文期刊>Vegetos: an international journal of plant research >Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb
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Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb

机译：体细胞胚胎发生和植株再生竹板jaeschkeana Vatke:药用的威胁草

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摘要

An efficient in vitro propagation protocol for an important medicinal endangered herb Ferula jaeschkeana has been developed through somatic embryogenesis. 12 week old friable calli were derived from petiole explants on a medium supplemented with 2.0 mgl-1 2,4-D and 1.0 mgl-1 Kn and used as mother source for somatic embryogenesis. Different auxins (2,4-D, IBA, IAA and NAA) in different concentrations (0.2-4.0 mgl-1) was used to produce embryogenic callus. Eighty four percent calli produced globular embryos on a medium supplemented with 4.0 mgl-1 2,4-D. The globular embryo then mature on culture medium (Control) or supplemented with different addictives (auxin, proline and ABA), maturation was significantly affected by ABA, proline and number of subcultures with maximum number of mature embryo (32.1 ± 0.2) was formed on medium fortified with 2.0 mgl-1 proline. Mature embryos had two well-developed cotyledons and an elongated hypocotyl root axis. 78% of these embryos were transformed into plantlets when placed in culture medium fortified with 0.2 + 0.01 + 0.05 mgl-1 (BAP:IBA:GA3) respectively. Regenerated plants were hardened in vermiculite with a 23% survival rate. The regenerated plants showed normal diploid chromosome number (2n = 22) and leaf morphology was comparable to in vivo plant.
机译：一个有效的体外传播协议的重要的药用濒危草本教鞭通过体细胞jaeschkeana了胚胎发生。来自叶柄外植体介质补充2.0 mgl-1 2,4 - d和1.0 mgl-1Kn,作为母亲体细胞来源胚胎发生。不同浓度NAA) (0.2 - -4.0mgl-1)是用于生产胚胎发生的愈伤组织。百分之八十四愈伤组织产生球状胚胎与4.0 mgl-1中补充2,4 - d。介质(控制)或与不同的补充添加剂(生长素、脯氨酸和ABA),成熟明显受ABA、脯氨酸和最大数量的亚文化成熟胚(32.1±0.2)中形成的2.0强化mgl-1脯氨酸。有两个成熟的子叶和一个吗细长的下胚轴根轴。胚胎时转换成植株放置在培养基和0.2 +强化0.01 + 0.05 mgl-1 (BAP: IBA: GA3)。再生植物在蛭石硬存活率为23%。显示正常的二倍体染色体数目(2 n = 22)和叶形态与体内工厂。

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