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首页> 外文期刊>Vegetos: an international journal of plant research >Computational analysis of microarray-based gene expression profiling and unveiling the functional traits in the developmental phases of Glycine max seed
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Computational analysis of microarray-based gene expression profiling and unveiling the functional traits in the developmental phases of Glycine max seed

机译：基因芯片的计算分析表达分析和发布功能在大豆的发展阶段特征种子

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摘要

The Glycine max seed is an ultimate source of protein worldwide and plays the crucial role in crop development. This made us curious to insight the developmental stages of G. max seed at the genomic level with computational based microarray data analysis. The data analysis explored 10453 and 6085 significant DEGs from 0 to 4 mm vs. 12 to 16 mm and 4-8 mm vs. 8-12 mm stage, respectively and based on log2 FC, 407 & 2342 DEGs were regulating high expression and 340 & 1414 DEGs were low in expression atthe 0-4 mm vs. 12-16 mm and 4-8 mm vs. 8-12 mm stage, respectively. Gene symbols for DEGs has recognized and identified that, 7 DEGs from 0-4 mm vs. 12-16 mm stage were coding for miRNAs. Gene classification searched 2 & 4 highly enriched groups of genesfrom up-down regulated DEGs of 0-4 mm vs. 12-16 mm stage, respectively and 19 & 6 groups of genes from up-down regulated DEGs of 4-8 mm vs. 8-12 mm stage that were showing functional similarities. Analysis for functional characterization has found that38% & 36% DEGs were annotating for BP, 42%&67% DEGs were for CC and 53% & 46% DEGs were for MF in up-down regulated DEGs of 0-4 mm vs. 12-16 mm stage, respectively. For up-down regulated DEGs of 4-8 mm vs. 8-12 mm stage has drawn and 8% &10% DEGs were indicating BP, 4% & 3% for CC and 15% & 23%DEGs were annotating for MF, respectively. Top ten highly regulated genes with the ID 11998843, 11855572, 12189716, etc. from stage 0-4 mm vs. 12-16 mm were annotating 10 BP, 6 MF and 6 CC. While, up-regulated DEGs with the ID Gma.10969.1.S1_at, GmaAffx.92715.1.S1_s_at, etc. were annotating 19 MF, 7 BP and one term from CC for stage 4-8 mm vs. 8-12 mm. These analyses would be helpful in the further characterization of interesting candidate genes that involve in the seed development of G. max.
机译：大豆种子的最终来源蛋白质在世界范围内,扮演着至关重要的作用作物的发展。g . max种子的发育阶段基于计算的微阵列基因水平数据分析。和6085年重要度从0到4毫米和1216毫米和4 - 8毫米和阶段,8 - 12毫米分别基于log2 FC, 407 & 2342度调节高表达和340 &1414度低表达在0 - 4毫米vs。长达12 - 16毫米,4 - 8毫米和8 - 12毫米的阶段,分别。识别和确定,7从0 - 4度毫米和12到16毫米级编码microrna。基因分类搜索2 & 4高度丰富的genesfrom上下调节度0 - 4毫米和12到16毫米阶段,分别和19日& 6组基因上下调节度的4 - 8毫米和8 - 12毫米的阶段显示功能的相似之处。功能描述发现that38% &36%度被注解为基点,42% &67%度CC和53% & 46%度MF的吗上下调节度0 - 4毫米和12到16毫米阶段,分别。4 - 8毫米和8 - 12毫米阶段&10%引起和8%度是表示英国石油,4% & & CC为3%和15%分别23%度被注解为曼氏金融。10基因高度管制的ID 11998843,11855572、12189716等从阶段0 - 4毫米vs。12到16毫米注释10个基点,6 MF和6 CC。同时,差异度与IDGma.10969.1。注释19 MF,从CC 7英国石油(BP)和一个学期吗阶段4 - 8毫米和8 - 12毫米。将有助于进一步描述有趣的候选基因,参与的种子发展g . max。

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