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首页> 外文期刊>Vegetos: an international journal of plant research >Stenotrophomonas: a versatile diazotrophic bacteria from the rhizospheric soils of Western Himalayas and development of its liquid biofertilizer formulation
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Stenotrophomonas: a versatile diazotrophic bacteria from the rhizospheric soils of Western Himalayas and development of its liquid biofertilizer formulation

机译：Stenotrophomonas:多功能diazotrophic从西方的根际的土壤细菌喜马拉雅山脉和开发液态生物肥料配方

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Rhizosphere is a rich repository of plant growth promoting rhizobacteria (PGPR) which is a sustainable tool to increase crop productivity and maintain soil health. In this context, 43 isolates were obtained on Jensen’s medium from the rhizosphere ofTriticum aestivum, Zea mays, Solanum tuberosum, Aloe barbadensis and Bacopa monnieri grown in Palampur, (Himachal Pradesh) India. Out of these isolates, only six isolates (WT-A2, WT-A1, MZ-A2, PT-A1, PT-A3 and BM-A3) exhibited significantly higher nitrogenase activity (451.45, 441.58, 440.91, 444.02, 383.64 and 374.44 nmole C2H4 h-1 mg-1 protein) as compared to the reference strain of Azotobacter chroococum MTCC 446 (372.85 nmole C_2H_4 h~(-1) mg~(-1) protein). The isolate WT-A2 was the most efficient with respect to nitrogenase activity (451.45 nmole C_2H_4 h~(-1) mg~(-1) protein), indole acetic acid production (17.45 mug m~l(-1)), ammonia production and siderophore production. Isolate WT-A2 was identified as Stenotrophomonas rhizophila on the basis of morphological, biochemical and 16S rRNA sequence analysis. In order to prepare liquid bioinoculant formulation, survivability studies on S. rhizophila was carried out in four different liquid carriers (Compost Tea, Biogas slurry, Vermiwash and MinimalGrowth Medium) at room temperature (average maximum temp. was 23.83 °C and average minimum temp. was 11.91 °C). The results showed that S. rhizophila survived better in different liquid carriers (9.873 log cfu m~l(-1) in biogas slurry; 9.843 log cfu m~l(-1) in vermiwash; 9.163 log cfu m~l(-1) in minimal growth medium), and Compost Tea was the best carrier to support higher bacterial load (9.907 log cfu m~l(-1)) on 180th day of storage. The results are of practical importance as this (compost tea) liquid carrier could be used to produce liquid biofertilizer formulation. Also, S. rhizophila could be a potential biofertilizer candidate as it posses multifarious plant growth promoting traits.
机译：根际是一个丰富的植物生长库这是一个促进rhizobacteria (PGPR)可持续的工具来提高农作物产量并保持土壤健康。Jensen的介质隔离了的根际ofTriticum aestivum,玉米,茄属植物tuberosum,索芦荟和植入monnieri生长在Palampur(喜马偕尔邦)印度。(WT-A1 WT-A2 MZ-A2、PT-A1 PT-A3和BM-A3)表现出明显高于固氮酶活动(451.45,441.58,440.91,444.02,383.64和374.44 h - nmole C2H4 mg-1蛋白质)相对于参考的固氮菌chroococum MTCC 446(372。85 nmole C_2H_4 h ~ (- 1)毫克~(1)蛋白质)。有效的对固氮酶活性(451.45 nmole C_2H_4 h ~(1)毫克~(1)蛋白质),吲哚乙酸生产(17.45杯m ~ l(1)),氨生产和含铁细胞生产。Stenotrophomonas rhizophila的基础上形态学、生物化学和16 s rRNA序列分析。制定、生存能力的研究。rhizophila进行了四个不同的液体载体(堆肥茶,沼气浆,Vermiwash MinimalGrowth媒介)的房间温度(平均最大temp. 23.83°C和平均最低temp. 11.91°C)。结果表明,s . rhizophila更好地存活下来在不同的液体载体(9.873日志cfu在沼气泥浆m ~ l (1);在vermiwash;生长培养基),堆肥茶是最好的运营商支持更高的细菌负荷(9.907日志cfu m ~ l(1))在第180天的存储。结果的实际意义(堆肥茶)液体载体可用于生产液体生物肥料配方。美国rhizophila可能是一个潜在的生物肥料候选人拥有各式各样的植物生长促进特征。

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