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首页> 外文期刊>Vegetos: an international journal of plant research >Cloning of mature pomegranate (Punica granatum) cv. Jalore seedless via in vitro shoot production and ex vitro rooting
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Cloning of mature pomegranate (Punica granatum) cv. Jalore seedless via in vitro shoot production and ex vitro rooting

机译：克隆成熟的石榴(石榴)简历。和前男友体外加油

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摘要

A novel approach of in vitro shoot amplification and ex vitro rooting for cloning of mature plants of Punica granatum cv. Jalore seedless/soft-seeded has been defined. Surface-sterilized nodal shoots were cultured for axillary meristem activation, budbreaking and shoot amplification. Multiple shoots differentiated by bud breaking from 82.8% of the explants on Murashige and Skoog (Physiol Plant 15:473-497, 1962) MS medium with 13.32 pM 6-benzylaminopurine (BAP). These were amplified by subculturing of fresh shoots and by repeated transfer of mother explants on MS medium + BAP or Kinetin (Kin)/Furfurylaminopurine (FAP) in combination with Indole acetic acid (IAA) or a-naphthalene acetic acid (NAA). MS medium with BAP 2.22 pM + IAA 0.57 pM was found to be most suitable for shoot (14.2 ± 1.03 shoots per culture vessel) multiplication. On half-strength MS medium with 9.84 pM of Indole butyric acid (IBA) and 16.65 pM of activated charcoal (AC), 72.9% of the micro-shoots rooted. Alternatively, base(s) of the micro-shoots treated with 1476 pM of IBA for 300 s. and transplanted on autoclaved soilrite (moistened with one-fourth strength of MS macro-salts) in glass bottles (420 ml; 70 mm diameter x 130 mm height). More than 85% of the IBA-treated shoots rooted ex vitro in a greenhouse. Ex vitro rooting of cloned shoots is a new approach for propagation of pomegranate. The process described is different and superior to all the described tissue culture methods for cloning of pomegranate. This is faster, cost-effective and saves resources enabling acclimatization/hardening with ease while minimizing microbial contamination, thus ensuring quick field transfer of hardened plantlets. This can be applied for mass and clonal propagation of selected genotypes andalso for long-term conservation of germplasm of P. granatum.
机译：体外射放大的新方法和前男友体外支持克隆成熟的植物无籽/ soft-seeded被定义。Surface-sterilized节芽培养了腋分生组织激活,发芽射放大。分化的芽打破82.8%的外植体Murashige和斯库(杂志女士15:473 - 497,1962)中以13.32点6-benzylaminopurine (BAP)。通过接种新鲜芽和重复母亲外植体在MS培养基+软面包卷或转让激动素(亲属)/ Furfurylaminopurine (FAP)与吲哚乙酸(IAA)或组合a-naphthalene乙酸(NAA)。BAP + IAA 0.57下午2.22点被发现适合拍摄(14.2±1.03芽培养容器)乘法。女士中吲哚丁酸的9.84点(IBA)和16.65点的活性炭(AC),micro-shoots扎根的72.9%。基地(s)的micro-shoots治疗1476点热压处理过的IBA 300 s和移植soilrite(四分之一的滋润女士macro-salts)玻璃瓶(420毫升;直径130毫米x高度)。IBA-treated拍摄的前女友体外温室。石榴的传播的新方法。描述的过程是不同的,优越的所有的组织培养方法进行了描述克隆的石榴。使成本效益,节省资源适应环境轻松/硬化减少微生物污染,从而保证快速硬化植株转移。可以申请质量和无性繁殖的选择基因型以及长期保护的p . granatum种质。

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