首页> 外文期刊>Vegetos: an international journal of plant research >Physiological and gene expression studies of selected Zea mays L. and Pennisetum glaucum (L.) R. Br. genotypes to simulated drought stress condition
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Physiological and gene expression studies of selected Zea mays L. and Pennisetum glaucum (L.) R. Br. genotypes to simulated drought stress condition

机译:生理和基因表达的研究选择的玉蜀黍l和狼尾草glaucum (l)r . Br。条件

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Drought is a major environmental stress that significantly obstructs productivity of various crops worldwide. Among several remedial methods to conquer this abiotic stress, use of resistant cultivars is most preferred. This study compares the responsein selected genotypes of pearl millet and maize under a progressive drought stress conditions at specific intervals. Three genotypes (IP14599, IP14787 and LRNO3) of pearl millet recorded relatively higher water content (RWC) than the three genotypes (DTSYN11, LRN03 and LRIO1) of maize. Leaf water potential (W_L), leaf osmotic potential (W_n), leaf turgor potential (W_p) and PSII were measured in harvests to attain comparative observations. Furthermore, to correlate these results expression of three genes were measured. It was observed that decreased over time and W_p recorded a decrease with WLat a higher rate in maize compared to pearl millet. A more declining trend in maximum fluorescence (AF/Fm') and electron transport rate (ETR) in LRIOI, LRNO1 and DTSYN11 was recorded compared to IP14599, IP14787 and LRNO3. The study of gene CBF in leaves and roots revealed it’s responsiveness to drought in genotypes of pearl millet IP14599, LRNO3 and IP14787 while it was absent in maize genotypes LRNO1 and LRIO1, with the exception of DTSYN11. Expression of RubSc gene showed a noteworthy decline in reactive oxygen species in the genotypes IP14599, LRNO3, DTSYN11 and IP14787, while a marked increase was observed in LRNO1 and LRIO1. Likewise gene PIP2;3 were highly responsive to drought in pearl millet but not in maize, where they might support greater water transport. Overall, the results indicate remarkable activation of mRNA expression of these genes under drought stress which provides the resistance against drought.
机译:干旱是一个主要的环境压力大大阻碍生产力的各种全球作物。征服这非生物压力,耐药的使用品种是最优先的。珍珠谷子的responsein选定的基因型逐渐干旱胁迫下和玉米条件以特定的间隔。(IP14599 IP14787和LRNO3)的珍珠粟记录相对较高的含水量(RWC)比三个基因型(DTSYN11 LRN03和LRIO1)的玉米。渗透势(W_n),叶膨的潜力(W_p)和PSII测量在收成达到比较观察。这些结果三个基因的表达相关被测量。随着时间的推移和WLat W_p记录减少更高的利率相比,玉米珍珠谷子。更多的最大荧光呈下降趋势(AF / Fm”)和电子传递速率(ETR)LRIOI, LRNO1 DTSYN11记录相比IP14599, IP14787 LRNO3。在根和叶显示响应能力干旱在珍珠谷子IP14599基因型,在玉米LRNO3和IP14787时缺席基因型LRNO1 LRIO1,除了DTSYN11。值得注意的活性氧在下降基因型IP14599、LRNO3 DTSYN11和观察IP14787,而显著增加LRNO1 LRIO1。在珍珠粟而不是回应的干旱玉米,他们可能会支持更多的水交通工具。这些显著激活mRNA的表达干旱胁迫下的基因提供了抵抗干旱。

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