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首页> 外文期刊>EMBO Journal >A dual role of H4K16 acetylation in the establishment of yeast silent chromatin.
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A dual role of H4K16 acetylation in the establishment of yeast silent chromatin.

机译：H4K16乙酰化的双重角色建立酵母沉默染色质。

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摘要

Discrete regions of the eukaryotic genome assume heritable chromatin structure that is refractory to transcription. In budding yeast, silent chromatin is characterized by the binding of the Silent Information Regulatory (Sir) proteins to unmodified nucleosomes. Using an in vitro reconstitution assay, which allows us to load Sir proteins onto arrays of regularly spaced nucleosomes, we have examined the impact of specific histone modifications on Sir protein binding and linker DNA accessibility. Two typical marks for active chromatin, H3K79(me) and H4K16(ac) decrease the affinity of Sir3 for chromatin, yet only H4K16(ac) affects chromatin structure, as measured by nuclease accessibility. Surprisingly, we found that the Sir2-4 subcomplex, unlike Sir3, has higher affinity for chromatin carrying H4K16(ac). NAD-dependent deacetylation of H4K16(ac) promotes binding of the SIR holocomplex but not of the Sir2-4 heterodimer. This function of H4K16(ac) cannot be substituted by H3K56(ac). We conclude that acetylated H4K16 has a dual role in silencing: it recruits Sir2-4 and repels Sir3. Moreover, the deacetylation of H4K16(ac) by Sir2 actively promotes the high-affinity binding of the SIR holocomplex.
机译：离散的真核基因组区域承担可遗传的染色质结构耐火材料转录。染色质的特点是绑定的无声的信息监管(先生)的蛋白质未经修饰的核小体。调整分析,这使我们能够加载先生蛋白质阵列均匀间隔的核小体,我们检查的影响特定的组蛋白修饰在蛋白质爵士绑定和链接器DNA可访问性。标志着活跃的染色质,H3K79(我)H4K16 (ac)减少Sir3的亲和力染色质,然而只有H4K16 (ac)影响染色质结构,以核酸酶的可访问性。令人惊讶的是,我们发现Sir2-4复形,与Sir3不同,具有较高的亲和力染色质携带H4K16 (ac)。脱乙酰作用的H4K16 (ac)促进绑定的先生holocomplex Sir2-4的但不是异质二聚体。代替由H3K56 (ac)。乙酰化H4K16沉默中起着双重作用:它新兵Sir2-4和排斥Sir3。脱乙酰作用的H4K16 Sir2积极(ac)促进的高亲和性结合先生holocomplex。

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