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CTX: Exploring new alternatives in host factor-mediated filamentous phage replications

机译：在主机CTX:探索新的替代factor-mediated丝状噬菌体复制

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摘要

For a long time Ff phages from Escherichia coli provided the majority of the knowledge about the rolling circle replication mechanism of filamentous phages. Host factors involved in coliphages replication have been fully identified. Based on these studies, the function of Rep protein as the accessory helicase directly implicated in filamentous phage replication was considered a paradigm. We recently reported that the replication of some filamentous phages from Vibrio cholerae, including the cholera toxin phage CTXf, depended on the accessory helicase UvrD instead of Rep. We also identified HU protein as one of the host factors involved in CTXf and VGJf replication. The requirement of UvrD and HU for rolling circle replication was previously reportedin some family of plasmids but had no precedent in filamentous phages. Here, we enrich the discussion of our results and present new preliminary data highlighting remarkable divergence in the lifestyle of filamentous phages.
机译：很长一段时间Ff从大肠杆菌噬菌体提供了大部分的知识滚圈的复制机制丝状噬菌体。大肠杆菌噬菌体复制已经完全识别。直接代表蛋白质作为附件解旋酶涉及丝状噬菌体复制认为是一个范例。一些丝状噬菌体的复制霍乱弧菌,其中包括霍乱毒素噬菌体CTXf,依赖于辅助解旋酶UvrD相反我们还发现了胡的众议员蛋白质的宿主因素参与CTXf和VGJf复制。以前reportedin一些家庭的质粒没有丝状噬菌体的先例。丰富我们的讨论结果和礼物新的突出显著的初步数据散度的丝状的生活方式噬菌体。

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