Optogenetic Modulation and Reprogrammingof Bacteriorhodopsin-Transfected Human Fibroblastson Self-Assembled Fullerene C60 Nanosheets

Pei-Wen Luo; Hao-Wei Han; Chii-Shen YangLok Kumar ShresthaKatsuhiko ArigaShan-hui Hsu

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Optogenetic Modulation and Reprogrammingof Bacteriorhodopsin-Transfected Human Fibroblastson Self-Assembled Fullerene C60 Nanosheets

机译：Optogenetic调制和ReprogrammingofBacteriorhodopsin-Transfected人类Fibroblastson自组装富勒烯C60 Nanosheets

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Fullerenes have unique biocompatibility and photoelectric properties and are candidate materials for biomedical applications. Several cell membrane proteins in nature such as bacteriorhodopsin also have photoelectric properties. Highly expressible bacteriorhodopsin (HEBR) is a novel light-sensitive opsin that has the potential to trigger neural activities through optogenetic modulation. Here, HEBR plasmids are delivered to human fibroblasts and the cells are exposed to C60 fullerene self-assembled 2Dnanosheets. Results show that the above approach combined with light stimulation (3 s duration and three times per day) may promote repro-gramming and differentiation of human fibroblasts into neural-like cells in 7 d without any neural induction medium. The special photoelectric properties of fullerenes as culture substrates and transfected HEBR on the cell membrane may provide a new optogenetic platform for regulating the location (C60 nanosheet) and time (frequency of light illumination) for human fibroblasts to become neural-like cells, and may be applied to improve neural regeneration in the future.

机译：富勒烯具有独特的生物相容性光电特性和候选人材料的生物医学应用。细胞膜蛋白质等性质细菌视紫红质也有光电属性。(希伯来圣经)是一种新型的光敏视蛋白可能引发神经活动通过optogenetic调制。质粒是人类成纤维细胞和交付细胞暴露于C60富勒烯自组装2 dnanosheets。上述方法结合光刺激(3 s持续时间和三次天)可能促进repro-gramming和人类成纤维细胞的分化没有任何神经neural-like 7 d细胞感应介质。富勒烯在文化基质的性质和转染细胞膜上的希伯来圣经提供一种新的optogenetic监管平台位置(C60 nanosheet)和时间(频率人类成纤维细胞的光照明)成为neural-like细胞,并可能被应用到在未来提高神经再生。

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《Advanced Biosystems》 |2019年第2期|1800254/1-1800254/13|共13页
作者
Pei-Wen Luo; Hao-Wei Han; Chii-Shen YangLok Kumar ShresthaKatsuhiko ArigaShan-hui Hsu;
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作者单位

Department of Biochemical Science and TechnologyNational Taiwan UniversityTaipei 10617, Taiwan, Republic of China;

Supermolecules GroupWPI Center for Materials Nanoarchitectonics (MANA)National Institute for Materials Science1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan;

Institute ofPolymer Science and EngineeringNational Taiwan UniversityTaipei 10617, Taiwan, Republic of China;

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正文语种英语
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Cell Membrane Proteins; Photoelectric properties; Nerve RegenerationFibroblastFullerenesfullerene C60BacteriorhodopsinsNanosheets;

机译：细胞膜蛋白质;光电属性;神经C60BacteriorhodopsinsNanosheets;

Optogenetic Modulation and Reprogrammingof Bacteriorhodopsin-Transfected Human Fibroblastson Self-Assembled Fullerene C60 Nanosheets

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