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Application of bacteriophages for detection of foodborne pathogens

机译：应用噬菌体检测食源性病原体

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摘要

Bacterial contamination of food products presents a challenge for the food industry and poses a high risk for the consumer; Despite increasing awareness and improved hygiene measures; foodborne pathogens remain a threat for public health, and novel methods for detection of these organisms are needed. Bacteriophages represent ideal tools for diagnostic assays because of their high target cell specificity, inherent signal-amplifying properties, easy and inexpensive production, and robustness. every stage of the phage lytic multiplication cycle, from the initial recognition of the host cell to the final lysis event, may be harnessed in several ways for the purpose of bacterial detection. Besides intact phage particles, phage-derived affinity moleculessuch as cell wall binding domains and receptor binding proteins can serve for this purpose. This review provides an overview of existing phage-based technologies for detection of foodborne pathogens, and highlights the most recent developments in this field, with particular emphasis on phage-based biosensors.
机译：细菌污染的食品礼物食品行业面临的挑战和构成高消费者的风险;认识和改善卫生措施;食源性病原体为公众仍然是一个威胁健康,并为检测的新方法生物是必要的。诊断化验,因为的理想工具他们的靶细胞特异性高,固有的signal-amplifying属性,简单便宜的生产,和鲁棒性。阶段的噬菌体裂解增殖周期,从最初的识别的宿主细胞最后消散事件,可以利用几个方面对细菌的目的检测。phage-derived亲和力moleculessuch细胞壁绑定域名和受体结合蛋白为这一目的服务。现有phage-based技术的概述检测食源性病原体,并突出最近在这个领域发展,特别强调phage-based生物传感器。

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