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Inactivation of Streptomyces phage C31 by 405 nm light

机译：失活的链霉菌属噬菌体C31 405海里光

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Exposure to narrowband violet-blue light around 405 nm wavelength can induce lethal oxidative damage to bacteria and fungi, however effects on viruses are unknown. As photosensitive porphyrin molecules are involved in the microbicidal inactivation mechanism, and since porphyrins are absent in viruses, then any damaging effects of 405 nm light on viruses might appear unlikely. This study used the bacteriophage C31, as a surrogate for non-enveloped double-stranded DNA viruses, to establish whether 405nm light can induce virucidal effects. Exposure of C31 suspended in minimal media, nutrient-rich media, and porphyrin solution, demonstrated differing sensitivity of the phage. Significant reductions in phage titer occurred when exposed in nutrient-richmedia, with ~3-, 5- and 7-log10 reductions achieved after exposure to doses of 0.3, 0.5 and 1.4 kJ/cm2, respectively. When suspended in minimal media a 0.3-log10 reduction (P = 0.012) occurred after exposure to 306 J/cm2: much lower than the 2.7- and > 2.5-log10 reductions achieved with the same dose in nutrient-rich, and porphyrin-supplemented media, suggesting inactivation is accelerated by the photo-activation of light-sensitive components in the media. This study provides the first evidence of the interaction of narrowband 405 nm light with viruses, and demonstrates that viral susceptibility to 405 nm light can be significantly enhanced by involvement of exogenous photosensitive components. The reduced susceptibility of viruses in minimal media, compared with that of other microorganisms, provides further evidence that the antimicrobial action of 405 nm light is predominantly due to the photo-excitation of endogenous photosensitive molecules such as porphyrins within susceptible microorganisms.
机译：暴露于窄带紫蓝色光405纳米波长可以引起致命的氧化然而破坏细菌和真菌的影响病毒是未知的。分子参与了杀菌剂的失活机制,由于卟啉没有病毒,那么任何破坏性的影响405纳米病毒可能似乎不太可能。本研究使用噬菌体C31,代理为无包膜双链DNA病毒,建立405纳米光是否可以诱导杀病毒的效果。悬浮在最小的媒体,营养丰富的媒体,和卟啉的解决方案,演示了不同噬菌体的敏感性。在噬菌体效价时发生接触nutrient-richmedia ~ 3 - 5, 7-log10减少暴露剂量后实现分别为0.3、0.5和1.4 kJ / cm2。悬浮在媒体0.3 log10减少最小(P = 0.012)发生在暴露于306年J / cm2:远低于2.7 - > 2.5 log10减少了相同的剂量营养丰富,porphyrin-supplemented媒体,加速了失活光激活光敏组件媒体。交互的窄带光405海里病毒,表明病毒对405 nm光大大增强了参与外源性光敏组件。病毒的敏感性最小的媒体,与其他微生物相比,提供了进一步的证据表明,抗菌405纳米的光主要是由于行动内生的photo-excitation光敏在敏感分子卟啉等微生物。

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