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首页> 外文期刊>Clinical and vaccine immunology : >Anthrax Vaccine Precipitated Induces Edema Toxin-Neutralizing, Edema Factor-Specific Antibodies in Human Recipients
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Anthrax Vaccine Precipitated Induces Edema Toxin-Neutralizing, Edema Factor-Specific Antibodies in Human Recipients

机译：炭疽疫苗沉淀引起水肿Toxin-Neutralizing、水肿Factor-Specific抗体在人类接受者

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Edema toxin (ET), composed of edema factor (EF) and protective antigen (PA), is a virulence factor of Bacillus anthracis that alters host immune cell function and contributes to anthrax disease. Anthrax vaccine precipitated (AVP) contains low but detectable levels of EF and can elicit EF-specific antibodies in human recipients of AVP. Active and passive vaccination of mice with EF can contribute to protection from challenge with Bacillus anthracis spores or ET. This study compared humoral responses to ET in recipients of AVP (n = 33) versus anthrax vaccine adsorbed (AVA; n = 66), matched for number of vaccinations and time postvaccination, and further determined whether EF antibodies elicited by AVP contribute to ET neutralization. AVP induced higher incidence (77.8%) and titer (229.8 +/- 58.6) of EF antibodies than AVA (4.2% and 7.8 +/- 8.3, respectively), reflecting the reported low but detectable presence of EF in AVP. In contrast, PA IgG levels and ET neutralization measured using a luciferase-based cyclic AMP reporter assay were robust and did not differ between the two vaccine groups. Multiple regression analysis failed to detect an independent contribution of EF antibodies to ET neutralization in AVP recipients; however, EF antibodies purified from AVP sera neutralized ET. Serum samples from at least half of EF IgG-positive AVP recipients bound to nine decapeptides located in EF domains II and III. Although PA antibodies are primarily responsible for ET neutralization in recipients of AVP, increased amounts of an EF component should be investigated for the capacity to enhance next-generation, PA-based vaccines.
机译：水肿毒素(ET),由水肿因子(EF)和保护性抗原(PA),是一种毒性因素改变主机的炭疽杆菌免疫细胞功能和有助于炭疽疾病。但可检测水平的EF和可以包含低引起人类接受者EF-specific抗体AVP。与EF有助于保护与炭疽杆菌孢子或等挑战。本研究比较体液反应等接受者的AVP (n = 33)与炭疽疫苗吸附(阿瓦;接种疫苗和时间postvaccination,进一步确定EF抗体引起由avon有助于中和。诱导更高的发病率(77.8%)和效价(229.8+ / - 58.6)比艾娃EF抗体(4.2%和7.8分别为+ / - 8.3),反映出报告低,但可检测存在AVP的EF。相反,PA免疫球蛋白水平和ET中和测量使用luciferase-based环腺苷酸记者分析是健壮的和没有差别之间的两个疫苗组。回归分析未能检测到一个独立的贡献EF抗体等中和avon接受者;从avon血清中和抗体纯化等。血清样本至少一半的EFIgG-positive avon接受者绑定到9十肽位于EF域II和III。尽管PA抗体主要是负责任的在接受avon ET中和,大量的EF组件应该增加追究能力增强下一代,宾夕法尼亚的疫苗。

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