Palmitoylation of CD95 facilitates formation of SDS-stable receptor aggregates that initiate apoptosis signaling

Feig C; Tchikov V; Schutze SPeter ME

首页> 外文期刊>EMBO Journal >Palmitoylation of CD95 facilitates formation of SDS-stable receptor aggregates that initiate apoptosis signaling

【24h】

Palmitoylation of CD95 facilitates formation of SDS-stable receptor aggregates that initiate apoptosis signaling

机译：棕榈酰化的CD95促进形成启动SDS-stable受体聚集细胞凋亡信号

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Apoptosis signaling through CD95 (Fas/APO-1) involves aggregation and clustering of the receptor followed by its actin-dependent internalization. Internalization is required for efficient formation of the death-inducing signaling complex (DISC) with maximal recruitment of FADD, caspase-8/ 10 and c-FLIP occurring when the receptor has reached an endosomal compartment. The first detectable event during CD95 signaling is the formation of SDS-stable aggregates likely reflecting intense oligomerization of the receptor. We now demonstrate that these SDS-stable forms of CD95 correspond to very high molecular weight DISC complexes (hiDISC) and are the sites of caspase-8 activation. hiDISCs are found both inside and outside of detergent-resistant membranes. The formation of SDS-stable CD95 aggregates involves palmitoylation of the membrane proximal cysteine 199 in CD95. Cysteine 199 mutants no longer form SDS-stable aggregates, and inhibition of palmitoylation reduces internalization of CD95 and activation of caspase-8. Our data demonstrate that SDS-stable forms of CD95 are the sites of apoptosis initiation and represent an important early step in apoptosis signaling through CD95 before activation of caspases.

机译：细胞凋亡信号通过CD95 (Fas / APO-1)包括聚合和集群的受体的actin-dependent紧随其后内化。death-inducing的有效形成信号复杂(盘)和最大的招聘FADD、caspase-8 / 10和c-FLIP时发生受体已达到一个endosomal隔间。CD95信号是SDS-stable的形成总量可能反映强烈齐聚反应的受体。证明这些SDS-stable CD95形式对应于高分子量盘配合物(hiDISC)和是caspase-8的场所激活。detergent-resistant外膜。形成SDS-stable CD95总量包括棕榈酰化膜的近半胱氨酸199年的CD95。SDS-stable聚集,抑制棕榈酰化减少CD95内化caspase-8和激活。SDS-stable CD95形式的网站细胞凋亡启动和代表一个重要细胞凋亡信号通过CD95早一步激活之前还存在。

著录项

来源
《EMBO Journal》 |2007年第1期|221-231|共11页
作者
Feig C; Tchikov V; Schutze SPeter ME;
展开▼
作者单位

Univ Chicago, Ben May Inst Canc Res, Chicago, IL 60637 USA;

Univ Hosp Schleswig Holstein, Inst Immunol, Kiel, Germany;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类分子生物学;
关键词
palmitoylation; Internalization; Activationlipid raftDeath Domain ReceptorsCD95 AntigensReceptor BindingProteinReceptorCaspase 8;

机译：棕榈酰化;国际化;ActivationlipidraftDeath a . ReceptorsCD95 AntigensReceptorBindingProteinReceptorCaspase 8;

相似文献

外文文献
中文文献

1. Alisol B 23-acetate-induced HepG2 hepatoma cell death through mTOR signaling-initiated G1 cell cycle arrest and apoptosis: A quantitative proteomic study [J] . Ji Xia, Oiang Luo, Shengbin Huang, 中国癌症研究（英文版） . 2019,第002期
2. Increased serum IL-10 in lupus patients promotes apoptosis of T cell subsets via the caspase 8 pathway initiated by Fas signaling [J] . Xiaofan Yang, Bin Sun, Huijuan Wang, 生物医学研究杂志（英文版） . 2015,第003期
3. Increased serum IL-IO in lupus patients promotes apoptosis of T cell subsets via the caspase 8 pathway initiated by Fas signaling [J] . Xiaofan Yang, Bin Sun, Huijuan Wang, 生物医学研究杂志：英文版 . 2015,第003期
4. Death receptor 5-mediated TNFR family signaling pathways modulate gamma-humulene-induced apoptosis in human colorectal cancer HT29 cells [O] . Lan, Y.H., Wu, Y.C., Wu, K.W., 2014

机译：Death receptor 5-mediated TNFR family signaling pathways modulate gamma-humulene-induced apoptosis in human colorectal cancer HT29 cells

Palmitoylation of CD95 facilitates formation of SDS-stable receptor aggregates that initiate apoptosis signaling

摘要

著录项

相似文献

相关主题

期刊订阅