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首页> 外文期刊>EMBO Journal >Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks
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Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks

机译：针对工程的线虫后基因组Mos1-triggered染色体休息时间

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摘要

The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template. Consequently, mutations engineered in the transgene could be copied to a specific locus at high frequency. This pathway was further characterized to develop an efficient tool - called MosTIC - to manipulate the C. elegans genome. Analysis of DSB repair during MosTIC experiments demonstrated that DSBs could also be sealed by end-joining in the germ line, independently from the evolutionarily conserved Ku80 and ligase IV factors. In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome.
机译：果蝇元素Mos1 II级转座子,这举动,“剪切和粘贴”机理和实验可以动员秀丽隐杆线虫种系。触发Mos1确定的切除插入创建染色体断裂了网站和进一步操作破碎的位点。双链断裂修复(双边带)通过使用转基因基因转换包含序列同源的打破染色体区域作为修复模板。因此,突变的工程转基因可能会被复制到一个特定的轨迹高频率。的特点,开发一个有效的工具叫MosTIC——操纵秀丽隐杆线虫基因组。实验表明,双边带也可以盖章end-joining生殖细胞系,独立的进化Ku80和连接酶IV因素。公开Mos1插入图书馆目前,生成MosTIC将提供一个通用工具来定制秀丽隐杆线虫的基因组。

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