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首页> 外文期刊>EMBO Journal >Proteasome substrate degradation requires association plus extended peptide
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Proteasome substrate degradation requires association plus extended peptide

机译：蛋白酶体基质降解需要协会+扩展肽

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摘要

To determine the minimum requirements for substrate recognition and processing by proteasomes, the functional elements of a ubiquitin-independent degradation tag were dissected. The 37-residue C-terminus of ornithine decarboxylase (cODC) is a native degron, which also functions when appended to diverse proteins. Mutating the cysteine 441 residue within cODC impaired its proteasome association in the context of ornithine decarboxylase and prevented the turnover of GFP-cODC in yeast cells. Degradation of GFP-cODC with C441 mutations was restored by providing an alternate proteasome association element via fusion to the Rpn10 proteasome subunit. However, Rpn10-GFP was stable, unless extended by cODC or other peptides of similar size. In vitro reconstitution experiments confirmed the requirement for both proteasome tethering and a loosely structured region. Therefore, cODC and degradation tags in general must serve two functions: proteasome association and a site, consisting of an extended peptide region, used for initiating insertion into the protease.
机译：确定的最低要求底物识别和处理水解酶的功能元素ubiquitin-independent降解标签是解剖。脱羧酶(cODC)是一个本地degron,同时当附加到不同的蛋白质的功能。441半胱氨酸突变cODC内残留物受损的蛋白酶体协会鸟氨酸脱羧酶和预防的营业额GFP-cODC酵母细胞。退化的GFP-cODC C441突变通过提供另一种蛋白酶体恢复通过融合到Rpn10协会元素蛋白酶体亚基。稳定,除非延长cODC或其他肽相似的大小。实验证实了这两个要求蛋白酶体拘束和松散的结构地区。一般必须服务于两个函数:蛋白酶体协会和一个网站,组成的一个扩展肽区域,用于启动插入蛋白酶。

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