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首页> 外文期刊>EMBO Journal >IL-6 and IL-12 specifically regulate the expression of Rab5 and Rab7 via distinct signaling pathways
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IL-6 and IL-12 specifically regulate the expression of Rab5 and Rab7 via distinct signaling pathways

机译：il - 6和il - 12专门规范Rab5和Rab7通过不同的表情信号通路

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Recent studies have shown that phagosome maturation depends on the balance between pro-inflammatory and anti-inflammatory cytokines, indicating that cytokine modulates phagosome maturation. However, the mechanism of cytokine-mediated modulation of intracellular trafficking remains to be elucidated. Here, we have shown that treatment of macrophages with IL-6 specifically induce the expression of Rab5 through the activation of extracellular signal-regulated kinase, whereas IL-12 exclusively upregulate the expression of Rab7 through the activation of p38 MAPK. We have cloned the 5'-flanking regions of the rab5c or rab7 into the promoterless reporter vector. Our results have shown that cells transfected with rab5c chimera are transactivated by IL-6, and IL-12 specifically transactivates cells containing rab7 chimera. Moreover, our results also show that IL-12 induces lysosomal transport, whereas IL-6 stimulates the fusion between early compartments in macrophages and accordingly modulates Salmonella trafficking and survival in macrophages. This is the first demonstration showing that cytokine differentially regulates endocytic trafficking by controlling the expression of appropriate Rab GTPase, and provides insight into the mechanism of cytokine-mediated regulation of intracellular trafficking.
机译：最近的研究表明,吞噬体成熟取决于之间的平衡促炎和抗炎细胞因子,表明细胞因子调节吞噬体成熟。cytokine-mediated调制的细胞内交易仍有待阐明。表明治疗巨噬细胞il - 6具体诱发Rab5的表达通过细胞外的激活signal-regulated激酶,而il - 12只上调Rab7的表达通过p38 MAPK的激活。克隆rab5c或5 '侧翼区域rab7 promoterless记者向量。结果表明,细胞转染rab5c嵌合体是transactivated il - 6,白介素专门transactivates细胞包含rab7妄想。还表明,il - 12引起溶酶体运输,而il - 6刺激早期之间的融合车厢在巨噬细胞和相应的行动调节沙门氏菌贩运和生存巨噬细胞。表明细胞因子调节不同内吞作用的交易通过控制适当的表达Rab GTPase和提供了洞察的机理cytokine-mediated调节细胞内人口贩卖。

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