Endogenous targets of RNA-directed DNA methylation and Pol IV in Arabidopsis

Huettel B; Kanno T; Daxinger LAufsatz WMatzke AJMatzke M

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Endogenous targets of RNA-directed DNA methylation and Pol IV in Arabidopsis

机译：内源性RNA-directed DNA甲基化的目标在拟南芥和波尔IV

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DRD1 is a SWI/SNF-like protein that cooperates with a plant-specific RNA polymerase, Pol IVb, to facilitate RNA-directed de novo methylation and silencing of homologous DNA. Screens to identify endogenous targets of this pathway in Arabidopsis revealed intergenic regions and plant genes located primarily in euchromatin. Many putative targets are near retrotransposon LTRs or other intergenic sequences that encode short RNAs, which might epigenetically regulate adjacent genes. Consistent with this, derepression of a solo LTR in drd1 and pol IVb mutants was accompanied by reduced cytosine methylation and transcriptional upregulation of neighboring sequences. The solo LTR and several other LTRs that flank reactivated targets are associated with euchromatic histone modifications but little or no H3K9 dimethylation, a hallmark of constitutive heterochromatin. By contrast, LTRs of retrotransposons that remain silent in the mutants despite reduced cytosine methylation lack euchromatic marks and have H3K9 dimethylation. We propose that DRD1 and Pol IVb establish a basal level of silencing that can potentially be reversed in euchromatin, and further reinforced in heterochromatin by other proteins that induce more stable modifications.

机译：DRD1是瑞士/ SNF-like蛋白质配合植物的RNA聚合酶,波尔IVb,司长委任促进RNA-directed从头甲基化同源基因的沉默。在拟南芥内源性目标的途径揭示了基因和植物基因间区域位于主要是在常染色质。逆转录转座子公升或其他附近的目标基因间序列编码rna,这可能epigenetically调节相邻吗基因。独奏LTR drd1和波尔IVb突变体是司长委任伴随着胞嘧啶甲基化和减少转录upregulation邻近的序列。,重新激活目标相关联常染色质的组蛋白修饰,但很少或没有H3K9 dimethylation,的一个特点结构异染色质。反转位子活动,保持沉默突变体尽管减少胞嘧啶甲基化所缺乏的常染色质的标志,H3K9 dimethylation。建议DRD1和波尔IVb建立基底司长委任水平可以的沉默常染色质逆转,进一步增强在其他蛋白质,从而诱导异染色质更稳定的修改。

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来源
《EMBO Journal》 |2006年第12期|2828-2836|共9页
作者
Huettel B; Kanno T; Daxinger LAufsatz WMatzke AJMatzke M;
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作者单位

Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria.;

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正文语种英语
中图分类分子生物学;分子生物学;
关键词
RNA Polymerase III; Retrotransposons; Genetic mutationsGene Expression ProfilingEuchromatinDRD1 geneMetabolism, plantChromatin ImmunoprecipitationHeterochromatinProtein Metabolism ProcessDNA MethylationArabidopsisupregultion;

机译：RNA聚合酶III,反转位子活动;遗传mutationsGene表达式ProfilingEuchromatinDRD1geneMetabolism, plantChromatinImmunoprecipitationHeterochromatinProtein新陈代谢ProcessDNAMethylationArabidopsisupregultion;

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专利

1. Escherichia coli DNA Polymerase IV (Pol IV), but Not Pol II, Dynamically Switches with a Stalled Pol III* Replicase [J] . Justin M. H. Heltzel, Robert W. Maul, David W. Wolff, Journal of bacteriology . 2012,第14期

机译：大肠杆菌DNA聚合酶IV（Pol IV），而不是Pol II，使用失速的Pol III *复制酶动态切换
2. Homology modeling of four Y-family, lesion-bypass DNA polymerases: The case that E. coli Pol IV and human Pol κ are orthologs, and E. coli Pol V and human Pol η are orthologs [J] . Chiu Hong Lee, Sushil Chandani, Edward L. Loechler Journal of molecular graphics & modelling . 2006,第1期

机译：四个Y家族，病变绕过DNA聚合酶的同源性建模：大肠杆菌Pol IV和人类Polκ是直系同源物，而大肠杆菌Pol V和人类Polη是直系同源物
3. Building a Structural Understanding of DNA Damage Repair a Mass Shift Analysis of DNA Ligase IV Regulation [C] . Martial Rey, Yaping Yu, Susan Lees-Miller, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：建立对DNA损伤修复的结构理解修复DNA连接酶IV调控的质量转变分析
4. Control of ribosomal RNA genes by maintenance and de novo methylation in Arabidopsis: Molecular genetics and evolution of the Pol IV/Pol V system. [D] . Tucker, Sarah Lynn. 2010

机译：在拟南芥中通过维持和从头甲基化来控制核糖体RNA基因：Pol IV / Pol V系统的分子遗传学和进化。
5. Escherichia coli DNA Polymerase IV (Pol IV) but Not Pol II Dynamically Switches with a Stalled Pol III* Replicase [O] . Justin M. H. Heltzel, Robert W. Maul, David W. Wolff, 2012

机译：大肠杆菌DNA聚合酶IV（Pol IV）但不是Pol II使用失速的Pol III *复制酶动态切换
6. An Effector of RNA-Directed DNA Methylation in Arabidopsis Is an ARGONAUTE 4-and RNA-Binding Protein [O] . He, X.J., Hsu, Y.F., Zhu, S.H., 2014

机译：an Effector of RNa-Directed DNa methylation in arabidopsis Is an aRGONaUTE 4-and RNa-Binding protein
7. Abstracts of the Conference on Mechanisms of DNA Repair and Mutagenesis on the 100. Anniversary of the Discovery of Polonium and Radium [R] . 1997

机译：关于发现pol和镭周年纪念的DNa修复和诱变机制会议摘要

Endogenous targets of RNA-directed DNA methylation and Pol IV in Arabidopsis

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