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首页> 外文期刊>EMBO Journal >A hyper-dynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-kappa B-dependent gene activity
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A hyper-dynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-kappa B-dependent gene activity

机译：一个hyper-dynamic promoter-bound之间的平衡和核质nf -κ二聚体控制B-dependent基因活性

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Because of its very high affinity for DNA, NF- kappa B is believed to make long- lasting contacts with cognate sites and to be essential for the nucleation of very stable enhanceosomes. However, the kinetic properties of NF- kappa B interaction with cognate sites in vivo are unknown. Here, we show that in living cells NF- kappa B is immobilized onto high- affinity binding sites only transiently, and that complete NF- kappa B turnover on active chromatin occurs in less than 30 s. Therefore, promoter- bound NF- kappa B is in dynamic equilibrium with nucleoplasmic dimers; promoter occupancy and transcriptional activity oscillate synchronously with nucleoplasmic NF- kappa B and independently of promoter occupancy by other sequence- specific transcription factors. These data indicate that changes in the nuclear concentration of NF- kappa B directly impact on promoter function and that promoters sample nucleoplasmic levels of NF- kappa B over a timescale of seconds, thus rapidly re- tuning their activity. We propose a revision of the enhanceosome concept in this dynamic framework.
机译：由于其非常高的亲和力为DNA, NF -κB被认为是持续时间较久接触同源网站和是至关重要的成核的enhanceosomes非常稳定。然而,NF -κB的动力学性质与体内同源网站交互未知的。κB是固定到高亲和力结合位点只有是暂时性的,完整的NF -κB营业额在活跃染色质发生在不到30年代。κB是在动态平衡核质二聚体;转录活动同步振荡与核质NF -κB和独立由其他序列——特定的启动子的入住率转录因子。NF -κ的核浓度的变化B对启动子功能,直接影响启动子样本核质水平的NF -κB /秒的时间尺度,从而迅速重新调整他们的活动。在这个动态的enhanceosome概念框架。

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