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首页> 外文期刊>EMBO Journal >Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla
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Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla

机译：空泡的顺序胞外分泌的大猴在肾上腺髓质囊泡

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Individual exocytic events in intact adrenal medulla were visualized by two- photon extracellular polar- tracer imaging. Exocytosis of chromaffin vesicles often occurred in a sequential manner, involving first vesicles located at the cell periphery and then those present deeper within the cytoplasm. Sequential exocytosis occurred preferentially at regions of the plasma membrane facing the intercellular space. The compound vesicles swelled to more than five times their original volume and formed vacuolar exocytic lumens as a result of expansion of intravesicular gels and their confinement within the lumen by the fusion pore and the narrow intercellular space. Such luminal swelling greatly promoted sequential exocytosis. The SNARE protein SNAP25 rapidly migrated from the plasma membrane to the membrane of fused vesicles. These data indicate that vesicles present deeper within the cytoplasm can be fusion ready like those at the cell periphery, and that swelling of exocytic lumens promotes assembly of the fusion machinery. We suggest the existence of two molecular configurations for fusion- ready states in Ca (2+)- dependent exocytosis.
机译：个人exocytic事件完整的肾上腺髓质被两个可视化——光子细胞外极-示踪成像。肾上腺髓质囊泡通常发生在一个顺序,包括第一个囊泡位于细胞边缘,然后这些现在更深的细胞质内。胞外分泌发生地区的优先细胞间面临的质膜空间。原来的5倍体积和形成空泡的exocytic流明的扩张intravesicular凝胶和监禁腔内的孔隙和融合狭窄的细胞间隙。极大地促进了顺序胞外分泌。从等离子体蛋白质SNAP25迅速迁移膜的膜囊泡融合。数据显示,囊泡深处细胞质可以融合喜欢那些做好准备细胞边缘,exocytic肿胀流明促进融合机械的装配。我们建议两个分子的存在配置融合——在Ca就绪状态(2 +) -胞外分泌的依赖。

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