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首页> 外文期刊>Chemical Engineering & Technology: Industrial Chemistry -Plant Equipment -Process Engineering -Biotechnology >Clusters of Phospholipid Vesicles as Platforms for Glucose Oxidase-Catalyzed Reaction in a Bubble-Column Bioreactor
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Clusters of Phospholipid Vesicles as Platforms for Glucose Oxidase-Catalyzed Reaction in a Bubble-Column Bioreactor

机译：集群的磷脂囊泡作为平台在葡萄糖Oxidase-Catalyzed反应泡罩塔生物反应器

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摘要

Clusters of negatively charged liposomes encapsulated with glucose oxidase were prepared in the presence of Ca2+ and used to catalyze the oxidation of glucose in an external loop airlift bubble column. The clusters exhibited higher catalytic activity compared to nonclustered glucose oxidase-containing liposomes (GOLs) when the liposome membranes were incorporated with cholesterol. The clusters were structurally altered in shear flow to give sufficient interfacial area accessible to glucose. The reactivity of GOL clusters could be modulated on the basis of lipid composition of the membranes which affected the mode of interaction among liposomes through Ca2+. Part of GOL clusters could be separated from the reaction mixture by centrifugation, which would be advantageous for reusing liposomal catalysts. The liposome clusters can be the platforms to regulate the catalytic performance of glucose oxidase in the airlift.
机译：带负电荷的脂质体的集群封装与葡萄糖氧化酶准备在Ca2 +的存在和用于催化氧化葡萄糖在外部循环空运泡沫柱。催化活性而非聚集葡萄糖oxidase-containing脂质体(高尔)时脂质体膜整合胆固醇。改变在剪切流给足够了界面区域的葡萄糖。反应性的高尔集群可以调制细胞膜的脂质成分的基础影响之间的相互作用的模式吗脂质体通过Ca2 +。可能从反应混合物中分离出来离心分离,这将是有利的重用脂质体的催化剂。集群可以调节的平台葡萄糖氧化酶的催化性能空运。

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