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首页> 外文期刊>Hepatology research: the official journal of the Japan Society of Hepatology >Crucial role of impaired Kupffer cell phagocytosis on the decreased Sonazoid-enhanced echogenicity in a liver of a nonalchoholic steatohepatitis rat model.
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Crucial role of impaired Kupffer cell phagocytosis on the decreased Sonazoid-enhanced echogenicity in a liver of a nonalchoholic steatohepatitis rat model.

机译：受损的枯氏细胞吞噬作用的至关重要的作用在减少Sonazoid-enhanced回声nonalchoholic性脂肪肝大鼠的肝脏模型。

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Aims: To evaluate the dynamics of Kupffer cell (KC) phagocytosis by performing both in vivo and in vitro studies using Sonazoid (GE Healthcare, Oslo) in a rat nonalcoholic steatohepatitis (NASH) model. Methods: Contrast enhanced ultrasonography (CEUS) was performed on a rat NASH model induced by a methionine choline deficient diet (MCDD) and control rats, and Sonazoid was used to measure the signal intensity in the liver parenchyma. The uptake of Sonazoid by the KCs was observed by intravital microscopy. Their phagocytic capability was evaluated in vitro using isolated and cultured KCs. The uptake of fluorescein isothiocyanate (FITC)-labeled latex beads was observed and quantitatively analyzed by flow cytometry. Results: In the MCDD group, liver parenchymal enhancement was reduced 20 min after the Sonazoid injection. Microscopic observation of the isolated and cultured KCs revealed that the number of phagocytosed Sonazoid microbubbles was significantly decreased. Confocal laser scanning microscopic (CLSM) observation showed a decrease in the uptake of the latex beads. A decreased phagocytic capacity in the MCDD group was suggested by the quantitative analysis using flow cytometry, as well as by intravital microscopy. Conclusions: CEUS with Sonazoid is a powerful evaluation tool to diagnose NASH from an early stage of the disease.
机译：目的:评价枯氏细胞的动力(KC)吞噬体内和表演体外研究使用Sonazoid(通用电气医疗集团,奥斯陆)大鼠非酒精性脂肪肝炎(纳什)模型。超声(对比增强超声)上执行一个老鼠纳什模型由蛋氨酸胆碱诱导缺乏饮食(MCDD)和控制老鼠,Sonazoid被用来测量信号强度肝实质。的;被活体的显微镜观察。其吞噬能力的评价使用分离和培养;体外。异硫氰酸荧光素(FITC)的标签乳胶珠子是观察和定量通过流式细胞术分析。组,肝实质增强减少20分钟后Sonazoid注入。观察分离和培养;显示的数量吞噬Sonazoid微气泡明显减少。共焦激光扫描显微镜(样品形貌)观察显示的吸收减少橡胶珠子。MCDD组的建议的使用流式细胞仪定量分析,如通过活体的显微镜。对比增强超声与Sonazoid是一个强大的评估工具诊断纳什的早期阶段疾病。

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